Tefito Utrasonic: "Ultrasonic Lysis and Cell Disruption"
Ko e fo'i lea ko e 'oku ne fakamatala'i e fakahoha'asi 'o e ngaahi holisi 'o e to'oto'o pe membranes 'a e lysis. Ultrasonic disruptors 'oku ha me'angaue falala'anga ke maumau'i 'a e ngaahi selo kae lava ke tau tukuange 'a e kalasiume intracellular hange ko DNA, polotini, organelles, mo phytochemicals. 'Oku faka'aonga'i 'a e ngaahi peau ma'olunga-malohi / ma'ulalo-tu'o lahi ha ki ultrasonic lysis (aka to'oto'o faingata'a), ke veteki 'a e membranes to'oto'o. Talu mei hono poupou'i 'e he ultrasonication 'a e fe'ave'aki pa'anga lahi, 'oku tukuange ai e naunau intracellular ki he totongi hono mo'ua takatakai ai. 'Oku ui ha lysate 'a e lolo 'oku 'i ai 'a e kakano 'o e fanga ki'i selo lysed.
To'oto'o lysis ko e 'uluaki sitepu 'i he to'oto'o fractionation, organelle li'ekina mo polotiini to'o e 'uli mo e fakama'a.
Laukonga lahi ange fekau'aki mo e to'oto'o ultrasonic disruptors mo e ultrasonic lysis!
peesi 'e 12 ki he tefito ko 'eni 'oku fakahaa'i atu:
Lysis toʻotoʻo ʻo bl21 ngaahi selo ʻe Ultrasonication
Ko e ngaahi selo BL21 ko ha mafasia ia ʻo e E. kololi ʻoku fakaʻaongaʻi lahi ʻi he fekumi laboratories, biotechnology mo e ngaahi meʻa fakapisinisi koeʻuhi ko ʻenau malava ke fakahaaʻi polotini lelei ʻaupito. Ultrasonic e fakahohaʻasi toʻotoʻo, lysis mo e ivi toʻo ʻuli ko e founga angamaheni ia…
https://www.hielscher.com/cell-lysis-of-bl21-cells-by-ultrasonication.htm
ʻOku pehe ʻe ultrasonic sipinga naʻe teuteuʻi maʻa ʻELISA As
ʻOku pehe ʻe ʻELISA ʻoku lahi hono fakaʻaongaʻi ki he diagnostics, fakatokanga ki he mahaki mo e mapuleʻi lelei (e.g. vakaiʻi ʻo e meʻakai allergens). Ko e sipinga ʻo e teuteu ultrasonic ko ha founga vave, falalaʻanga mo reproducible ia ke lyse toʻotoʻo mo fakamavaheʻi intracellular polotini, DNA, RNA…
https://www.hielscher.com/ultrasonic-sample-prep-for-elisa-assays.htm
Protocol ki he SARS-KoloV-2 Coronavirus fakamalohia mo Sonication
Ko e Hielscher VialTweeter ko ha ʻiuniti makehe ultrasonic fakavahaʻa-taʻu, ʻa ia ʻoku fakaʻaongaʻi ke inactivate ʻa e coronavirus SARS-KOV-2. ʻOku fakaʻata ʻe he VialTweeter ke teuteu ki ha sipinga ʻe 10 ʻi he taimi tatau pe pea ʻoku ʻi ai ʻa e ʻiuniti lelei taha ki he sipinga ngaue lahi.…
https://www.hielscher.com/protocol-for-sars-cov-2-coronavirus-inactivation-with-sonication.htm
Sipinga ʻo e teuteu mo e Ultrasonic VialTweeter
ʻE lava ke fie maʻu ha sipinga ʻo e teuteu kimuʻa pea toki ʻanalaiso ngaahi meʻa ngaahi founga kehekehe hange ko e homogenization, lysis, toʻo hingoa ʻo e polotini, DNA, RNA, organelles mo e ngaahi meʻa intracellular kehe, ʻo fakaleleiʻi mo degassing. Ko e VialTweeter ko ha meʻangaue makehe ultrasonic ia ʻoku ne teuteuʻi ha ngaahi sipinga lahi ʻo e tiupi…
https://www.hielscher.com/sample-preparation-with-the-ultrasonic-vialtweeter.htm
Biosynthetic hono ngaohi ʻo e huʻakau ʻa e tangata Oligosaccharides
Ko e biosynthesis ʻo e huʻakau fakaetangata ʻoku oligosaccharides (HMOs) ʻo fakafou ʻi fermentation pe enzymatic, ko ha ngaue ia ʻoku faingataʻa pea ʻoku faʻa fakatupu tolalo. Ultrasonication ʻa e lahi ʻo e paʻanga ʻi he substrate mo e ngaahi ʻa-toʻotoʻo ans fakaʻaiʻai ʻa e tupu mo e metabolism. ʻi heʻene pehee, kuo sonication ke fakamalohia…
https://www.hielscher.com/human-milk-oligosaccharides.htm
Ultrasonic lelei taha Dismembrators
ʻoku faʻa fakaʻaongaʻi ʻa e ultrasonic dismembrators ʻi he fale fakakemi, ʻi ʻolunga mo e ngaahi ngaueʻanga ke teuteuʻi homogeneous colloidal suspensions hange ko e emulsions mo e dispersions ʻaki ʻa e foʻi tulutaa pe ko e siʻi hono lalahi ʻi he ngaahi meʻa submicron mo e nano. ʻikai ngata ai, ʻoku fakaʻaongaʻi ʻa e ultrasonic dismembrators ki he fakahohaʻasi ʻo e meʻa toʻotoʻo,…
https://www.hielscher.com/most-efficient-ultrasonic-dismembrators.htm
Ultrasonic Disintegrators
Ko e me'a angamaheni ko e ngaue biotechnology 'a e ngaahi me'a 'oku fai pea ko e lahi taha hono faka'aonga'i 'oku kau ai 'a e processing selo mo e naunau intracellular. Ultrasonic 'oku lelei mo falala'anga e disintegrators to'oto'o, 'o a'u ki hono maumau'i e holisi pe membranes 'o e ngaahi selo, mammalian…
https://www.hielscher.com/ultrasonic-disintegrators.htm
Coronavirus (COVID-19, SARS-Kolov-2) mo Ultrasonics
Ko e Ultrasonication ko ha me'angaue malohi ia 'oku faka'aonga'i 'i he paiolosii, molecular e kemisitulii mo e biochemistry pea pehe ki hono ngaohi 'o e pharmaceuticals. 'Oku faka'aonga'i 'e piokalafi-saienisi 'a e ultrasonic homogenizers ki he ngaahi selo mo e konga polotini mo e ngaahi naunau intracellular kehe, 'a e pharma 'oku faka'aonga'i ultrasonics…
https://www.hielscher.com/coronavirus-covid-19-sars-cov-2-and-ultrasonics.htm
Ultrasonics 'i he fekumi 'i he vailasi
Ko e ultrasonic lysis mo hono to'o e 'uli ko ha founga pau mo taimi loloa ia ki hono fakamovetevete 'o e ngaahi selo pea mo hono tuku atu 'o e vailasi, viral polotini, DNA, mo e RNA. Ultrasonics 'i he Coronavirus fakatotolo ko hono to'o e 'uli 'o vailasi mei hono fetongi 'o e 'okani…
https://www.hielscher.com/ultrasonics-virus-research.htm
Ultrasonic to'o hingoa Cold-Water 'o e Mogrosides mei he monike fua
'Oku faka'aonga'i 'a e fua'i'akau (grosvenorii) ki hono fua mo tautefito ki hono komipauni mogroside V ko ha sweetener 'oku 'ikai ko ha me'a ia 'oku 'ikai ke 'iloa. Mogrosides. Ko e to'o hingoa ultrasonic ko ha founga faingofua, vave, reproducible pea mo falala'anga kuo ola lelei 'o ngaue 'aki ki hono ngaohi 'o ha sweetener 'oku 'ikai ma'u mei he patele…
https://www.hielscher.com/ultrasonic-cold-water-extraction-of-mogrosides-from-monk-fruit.htm
Fakalahi ultrasonically 'a e Partitioning 'a e toko tolu 'o e konga
Ko e konga-tolu partitioning (TPP) ko ha founga ia ke to'o 'aki, fakamavahevahe'i mo fakama'a e ngaahi me'a, e.g lipids, enzymes, polysaccharides mo e biomolecules kehe, mei he ngaahi me'a fakatu'asino. Na'e tokoni 'a e ultrasonically ki he konga tolu partitioning ke ma'olunga ange 'a e tait, pp. pea vave 'aupito hono fakatupulaki.…
https://www.hielscher.com/ultrasonically-enhanced-three-phase-partitioning.htm
Ultrasonic Psilocybin to'o e 'uli mei he fakamalu tevolo
Psilocybin is the active substance extracted from hallucinogenic mushrooms. Ultrasonic extraction is often used to extract the psilocybin efficiently from mushrooms. Ultrasonic extraction gives high psilocybin yield in a very rapid extraction time. Ultrasonic Isolation of Psilocybin Ultrasonic extraction…
https://www.hielscher.com/ultrasonic-psilocybin-extraction-from-mushrooms.htm