Tefito Utrasonic: "lysis"
Ko e fo'i lea ko e 'oku ne fakamatala'i e fakahoha'asi 'o e ngaahi holisi 'o e to'oto'o pe membranes 'a e lysis. Ultrasonic disruptors 'oku ha me'angaue falala'anga ke maumau'i 'a e ngaahi selo kae lava ke tau tukuange 'a e kalasiume intracellular hange ko DNA, polotini, organelles, mo phytochemicals. 'Oku faka'aonga'i 'a e ngaahi peau ma'olunga-malohi / ma'ulalo-tu'o lahi ha ki ultrasonic lysis (aka to'oto'o faingata'a), ke veteki 'a e membranes to'oto'o. Talu mei hono poupou'i 'e he ultrasonication 'a e fe'ave'aki pa'anga lahi, 'oku tukuange ai e naunau intracellular ki he totongi hono mo'ua takatakai ai. 'Oku ui ha lysate 'a e lolo 'oku 'i ai 'a e kakano 'o e fanga ki'i selo lysed.
To'oto'o lysis ko e 'uluaki sitepu 'i he to'oto'o fractionation, organelle li'ekina mo polotiini to'o e 'uli mo e fakama'a.
Laukonga lahi ange fekau'aki mo e to'oto'o ultrasonic disruptors mo e ultrasonic lysis!
peesi 'e 12 ki he tefito ko 'eni 'oku fakahaa'i atu:
Lysis toʻotoʻo ʻo bl21 ngaahi selo ʻe Ultrasonication
Ko e ngaahi selo BL21 ko ha mafasia ia ʻo e E. kololi ʻoku fakaʻaongaʻi lahi ʻi he fekumi laboratories, biotechnology mo e ngaahi meʻa fakapisinisi koeʻuhi ko ʻenau malava ke fakahaaʻi polotini lelei ʻaupito. Ultrasonic e fakahohaʻasi toʻotoʻo, lysis mo e ivi toʻo ʻuli ko e founga angamaheni ia…
https://www.hielscher.com/cell-lysis-of-bl21-cells-by-ultrasonication.htm
ʻOku pehe ʻe ultrasonic sipinga naʻe teuteuʻi maʻa ʻELISA As
ʻOku pehe ʻe ʻELISA ʻoku lahi hono fakaʻaongaʻi ki he diagnostics, fakatokanga ki he mahaki mo e mapuleʻi lelei (e.g. vakaiʻi ʻo e meʻakai allergens). Ko e sipinga ʻo e teuteu ultrasonic ko ha founga vave, falalaʻanga mo reproducible ia ke lyse toʻotoʻo mo fakamavaheʻi intracellular polotini, DNA, RNA…
https://www.hielscher.com/ultrasonic-sample-prep-for-elisa-assays.htm
Protocol ki he SARS-KoloV-2 Coronavirus fakamalohia mo Sonication
Ko e Hielscher VialTweeter ko ha ʻiuniti makehe ultrasonic fakavahaʻa-taʻu, ʻa ia ʻoku fakaʻaongaʻi ke inactivate ʻa e coronavirus SARS-KOV-2. ʻOku fakaʻata ʻe he VialTweeter ke teuteu ki ha sipinga ʻe 10 ʻi he taimi tatau pe pea ʻoku ʻi ai ʻa e ʻiuniti lelei taha ki he sipinga ngaue lahi.…
https://www.hielscher.com/protocol-for-sars-cov-2-coronavirus-inactivation-with-sonication.htm
Sipinga ʻo e teuteu mo e Ultrasonic VialTweeter
ʻE lava ke fie maʻu ha sipinga ʻo e teuteu kimuʻa pea toki ʻanalaiso ngaahi meʻa ngaahi founga kehekehe hange ko e homogenization, lysis, toʻo hingoa ʻo e polotini, DNA, RNA, organelles mo e ngaahi meʻa intracellular kehe, ʻo fakaleleiʻi mo degassing. Ko e VialTweeter ko ha meʻangaue makehe ultrasonic ia ʻoku ne teuteuʻi ha ngaahi sipinga lahi ʻo e tiupi…
https://www.hielscher.com/sample-preparation-with-the-ultrasonic-vialtweeter.htm
Biosynthetic hono ngaohi ʻo e huʻakau ʻa e tangata Oligosaccharides
Ko e biosynthesis ʻo e huʻakau fakaetangata ʻoku oligosaccharides (HMOs) ʻo fakafou ʻi fermentation pe enzymatic, ko ha ngaue ia ʻoku faingataʻa pea ʻoku faʻa fakatupu tolalo. Ultrasonication ʻa e lahi ʻo e paʻanga ʻi he substrate mo e ngaahi ʻa-toʻotoʻo ans fakaʻaiʻai ʻa e tupu mo e metabolism. ʻi heʻene pehee, kuo sonication ke fakamalohia…
https://www.hielscher.com/human-milk-oligosaccharides.htm
Ultrasonic lelei taha Dismembrators
ʻoku faʻa fakaʻaongaʻi ʻa e ultrasonic dismembrators ʻi he fale fakakemi, ʻi ʻolunga mo e ngaahi ngaueʻanga ke teuteuʻi homogeneous colloidal suspensions hange ko e emulsions mo e dispersions ʻaki ʻa e foʻi tulutaa pe ko e siʻi hono lalahi ʻi he ngaahi meʻa submicron mo e nano. ʻikai ngata ai, ʻoku fakaʻaongaʻi ʻa e ultrasonic dismembrators ki he fakahohaʻasi ʻo e meʻa toʻotoʻo,…
https://www.hielscher.com/most-efficient-ultrasonic-dismembrators.htm
Ultrasonic Disintegrators
Ko e me'a angamaheni ko e ngaue biotechnology 'a e ngaahi me'a 'oku fai pea ko e lahi taha hono faka'aonga'i 'oku kau ai 'a e processing selo mo e naunau intracellular. Ultrasonic 'oku lelei mo falala'anga e disintegrators to'oto'o, 'o a'u ki hono maumau'i e holisi pe membranes 'o e ngaahi selo, mammalian…
https://www.hielscher.com/ultrasonic-disintegrators.htm
Coronavirus (COVID-19, SARS-Kolov-2) mo Ultrasonics
Ko e Ultrasonication ko ha me'angaue malohi ia 'oku faka'aonga'i 'i he paiolosii, molecular e kemisitulii mo e biochemistry pea pehe ki hono ngaohi 'o e pharmaceuticals. 'Oku faka'aonga'i 'e piokalafi-saienisi 'a e ultrasonic homogenizers ki he ngaahi selo mo e konga polotini mo e ngaahi naunau intracellular kehe, 'a e pharma 'oku faka'aonga'i ultrasonics…
https://www.hielscher.com/coronavirus-covid-19-sars-cov-2-and-ultrasonics.htm
Ultrasonics 'i he fekumi 'i he vailasi
Ko e ultrasonic lysis mo hono to'o e 'uli ko ha founga pau mo taimi loloa ia ki hono fakamovetevete 'o e ngaahi selo pea mo hono tuku atu 'o e vailasi, viral polotini, DNA, mo e RNA. Ultrasonics 'i he Coronavirus fakatotolo ko hono to'o e 'uli 'o vailasi mei hono fetongi 'o e 'okani…
https://www.hielscher.com/ultrasonics-virus-research.htm
Ultrasonic to'o hingoa Cold-Water 'o e Mogrosides mei he monike fua
'Oku faka'aonga'i 'a e fua'i'akau (grosvenorii) ki hono fua mo tautefito ki hono komipauni mogroside V ko ha sweetener 'oku 'ikai ko ha me'a ia 'oku 'ikai ke 'iloa. Mogrosides. Ko e to'o hingoa ultrasonic ko ha founga faingofua, vave, reproducible pea mo falala'anga kuo ola lelei 'o ngaue 'aki ki hono ngaohi 'o ha sweetener 'oku 'ikai ma'u mei he patele…
https://www.hielscher.com/ultrasonic-cold-water-extraction-of-mogrosides-from-monk-fruit.htm
Fakalahi ultrasonically 'a e Partitioning 'a e toko tolu 'o e konga
Ko e konga-tolu partitioning (TPP) ko ha founga ia ke to'o 'aki, fakamavahevahe'i mo fakama'a e ngaahi me'a, e.g lipids, enzymes, polysaccharides mo e biomolecules kehe, mei he ngaahi me'a fakatu'asino. Na'e tokoni 'a e ultrasonically ki he konga tolu partitioning ke ma'olunga ange 'a e tait, pp. pea vave 'aupito hono fakatupulaki.…
https://www.hielscher.com/ultrasonically-enhanced-three-phase-partitioning.htm
Ultrasonic Psilocybin to'o e 'uli mei he fakamalu tevolo
Ko e Psilocybin 'oku ola malohi ia mei hallucinogenic fakamalu tevolo. 'Oku fa'a faka'aonga'i 'a e to'o hingoa ultrasonic ke to'o 'aki e 'uli psilocybin mei he fakamalu tevolo. 'Oku ultrasonic 'e he to'o hingoa 'a e ma'olunga psilocybin ne 'omi 'i ha taimi 'oku to'o vave. Ultrasonic mavahevahe 'a Psilocybin ultrasonic…
https://www.hielscher.com/ultrasonic-psilocybin-extraction-from-mushrooms.htm