Tema utilizar ya homogeneizadores ultrasónicos tejidos Hielscher
Before purifying or characterizing intracellular macromolecules such as proteins, organelles, enzymes, or active compounds, it is essential to employ an efficient method for tissue lysis and cell disintegration. Ultrasonication is a highly effective technique for cell preparation, quickly releasing intracellular materials into a buffer solution. The mechanical shear forces generated during ultrasonic tissue homogenization can be precisely adjusted to suit specific materials. This allows for gentle preparation of soft tissues or DNA/RNA shearing with milder sonication, as well as intense ultrasonic cavitation for destructive cell disruption (e.g., for nannochloropsis, yeast).
Below is a selection of tissues, cells, and other biological matter with recommended sonication protocols and guidelines for efficiently preparing your samples using an ultrasonic tissue homogenizer or cell crusher.
Lab ultra-sonicator UP200Ht (200W, 26kHz) for sample preparation tasks
How to Prepare your Lysates, Homogenates and Extracts from Biological Materials
'Bu̲ ya alfabético:
Acetobacter suboxydans
Actinomyces
ALP and LDH activity
Nt'ot'e ultrasónica:
Determination of ALP and LDH activity and protein content
Frozen cell samples were thawed for 20 min on ice, followed by cell lysis with PBS containing 1% Triton X-100 for 50 min on ice. During cell lysis each sample was sonicated for 1 min at 80 W with an ultrasonic processor UP100H (Hielscher Ultrasonics).
Ir nge ár hmä dispositivo:
UP100H
Referencia yá 'be̲fi ya nthoni:
Bernhardt, A. et al. (2008): Mineralised collagen—an artificial, extracellular bone matrix—improves osteogenic differentiation of bone marrow stromal cells.
Determination of ALP and LDH activity and protein content
Frozen cell samples were thawed for 20 min on ice, followed by cell lysis with PBS containing 1% Triton X-100 for 50 min on ice. During cell lysis each sample was sonicated for 1 min at 80 W with an ultrasonic processor UP100H (Hielscher Ultrasonics).
Ir nge ár hmä dispositivo:
UP100H
Referencia yá 'be̲fi ya nthoni:
Bernhardt, A. et al. (2008): Mineralised collagen—an artificial, extracellular bone matrix—improves osteogenic differentiation of bone marrow stromal cells.
Amorphous tricalcium phosphate (ATCP)
Nt'ot'e ultrasónica:
ATCP nano-particles, which are an exceptionally reactive precursor for the formation of hydroxyapatite, were dispersed in chloroform containing 5 % (w/w) Tween20 referred to PLGA using the Hielscher ultrasonic device UP400S at 320W for 5 min. applying pulsed intervals (50%) to allow for relaxation of the particles.
Ir nge ár hmä dispositivo:
UP400S
Referencia yá 'be̲fi ya nthoni:
Mohn, Dirk; Ege, Duygu; Feldman, Kirifl; Schneider, Oliver D.; Imfeld, Thomas; Boccaccini, Aldo R. (2014): Spherical calcium phosphate nanoparticle fillers allow polymer processing of bone fixation devices with high bioactivity. The Free Library 01 May 2010. 21 January 2014.
ATCP nano-particles, which are an exceptionally reactive precursor for the formation of hydroxyapatite, were dispersed in chloroform containing 5 % (w/w) Tween20 referred to PLGA using the Hielscher ultrasonic device UP400S at 320W for 5 min. applying pulsed intervals (50%) to allow for relaxation of the particles.
Ir nge ár hmä dispositivo:
UP400S
Referencia yá 'be̲fi ya nthoni:
Mohn, Dirk; Ege, Duygu; Feldman, Kirifl; Schneider, Oliver D.; Imfeld, Thomas; Boccaccini, Aldo R. (2014): Spherical calcium phosphate nanoparticle fillers allow polymer processing of bone fixation devices with high bioactivity. The Free Library 01 May 2010. 21 January 2014.
Antocianinas
Nt'ot'e ultrasónica:
Anthocyanins: di-glucosides, mono-glucoside, acylated monoglucosides and acylated di-glucosides of peonidin, malvidin, cyanidin, petunidin and delphinidin:Extraction from grape skin in 40 sec.; pH 5.0; ratio of material/extraction solvent of 1:6.
Ir nge ár hmä dispositivo:
UP100H
Anthocyanins: di-glucosides, mono-glucoside, acylated monoglucosides and acylated di-glucosides of peonidin, malvidin, cyanidin, petunidin and delphinidin:Extraction from grape skin in 40 sec.; pH 5.0; ratio of material/extraction solvent of 1:6.
Ir nge ár hmä dispositivo:
UP100H
Anthraquinones
Nt'ot'e ultrasónica:
Extraction of Anthraquinones from the roots of Morinda citrifolia
Ir nge ár hmä dispositivo:
UP400S
Extraction of Anthraquinones from the roots of Morinda citrifolia
Ir nge ár hmä dispositivo:
UP400S
Apricot seeds
Nt'ot'e ultrasónica:
Ultrasonic pre-treatment before extracting oil from the seeds of Jatropha curcas L. to improve extraction.
Ir nge ár hmä dispositivo:
UP400S
Ultrasonic pre-treatment before extracting oil from the seeds of Jatropha curcas L. to improve extraction.
Ir nge ár hmä dispositivo:
UP400S
Artemisia selengensis Turcz
Nt'ot'e ultrasónica:
Extraction of Rutin (1.0g milled sample in 30 mL methanol) at 25°C in 5-10 min.
Ir nge ár hmä dispositivo:
UP50H
Extraction of Rutin (1.0g milled sample in 30 mL methanol) at 25°C in 5-10 min.
Ir nge ár hmä dispositivo:
UP50H
Aspergillus flavus
Nt'ot'e ultrasónica:
Ultrasonic inactivation of Aspergillus flavus in Sabouraud growth medium
Ir nge ár hmä dispositivo:
UP200S
Ultrasonic inactivation of Aspergillus flavus in Sabouraud growth medium
Ir nge ár hmä dispositivo:
UP200S
B. sphaericus / Bacillus sphaericus
Bacillus anthracis sterne 34F2 spores
Nt'ot'e ultrasónica:
Ultrasonic removal of Bacillus anthracis sterne 34F2, Bacillus cereus ATCC 21281, and Bacillus thuringiensis ATCC 33680 spores. The use of 150 ppm of sodium hypochlorite along with ultrasound did lead to spore inactivation.
Ir nge ár hmä dispositivo:
UP200S at 100% amplitude
Referencia yá 'be̲fi ya nthoni:
Pamarthi, S.R. et al.: Effectiveness of Ultrasound in Desoiling Bacillus spp spores Embedded in Complex Food Matrices Attached to Various Contact Surfaces.
Ultrasonic removal of Bacillus anthracis sterne 34F2, Bacillus cereus ATCC 21281, and Bacillus thuringiensis ATCC 33680 spores. The use of 150 ppm of sodium hypochlorite along with ultrasound did lead to spore inactivation.
Ir nge ár hmä dispositivo:
UP200S at 100% amplitude
Referencia yá 'be̲fi ya nthoni:
Pamarthi, S.R. et al.: Effectiveness of Ultrasound in Desoiling Bacillus spp spores Embedded in Complex Food Matrices Attached to Various Contact Surfaces.
Bacillus cereus ATCC 21281 spores
Nt'ot'e ultrasónica:
Ultrasonic removal of Bacillus anthracis sterne 34F2, Bacillus cereus ATCC 21281, and Bacillus thuringiensis ATCC 33680 spores. The use of 150 ppm of sodium hypochlorite along with ultrasound did lead to spore inactivation.
Ir nge ár hmä dispositivo:
UP200S at 100% amplitude
Referencia yá 'be̲fi ya nthoni:
Pamarthi, S.R. et al.: Effectiveness of Ultrasound in Desoiling Bacillus spp spores Embedded in Complex Food Matrices Attached to Various Contact Surfaces.
Ultrasonic removal of Bacillus anthracis sterne 34F2, Bacillus cereus ATCC 21281, and Bacillus thuringiensis ATCC 33680 spores. The use of 150 ppm of sodium hypochlorite along with ultrasound did lead to spore inactivation.
Ir nge ár hmä dispositivo:
UP200S at 100% amplitude
Referencia yá 'be̲fi ya nthoni:
Pamarthi, S.R. et al.: Effectiveness of Ultrasound in Desoiling Bacillus spp spores Embedded in Complex Food Matrices Attached to Various Contact Surfaces.
Bacillus subtilis
Nt'ot'e ultrasónica:
High power, low frequency ultrasound in low volumes of bacterial suspension results in a continuous reduction in bacterial cell numbers i.e. the kill rate predominates. In larger volumes, sonication results in an initial rise in cell numbers suggesting declumping of the bacteria but this initial rise then falls as the declumping finishes and the kill rate becomes more important.
Ir nge ár hmä dispositivo:
UP200St
Referencia yá 'be̲fi ya nthoni:
Joyce, E.; Phull, S. S.; Lorimer, J. P.; Mason, T. J. (2003): The development and evaluation of ultrasound for the treatment of bacterial suspensions. A study of frequency, power and sonication time on cultured Bacillus species. Ultrason. Sonochem. 10/2003. pp. 315-318.
High power, low frequency ultrasound in low volumes of bacterial suspension results in a continuous reduction in bacterial cell numbers i.e. the kill rate predominates. In larger volumes, sonication results in an initial rise in cell numbers suggesting declumping of the bacteria but this initial rise then falls as the declumping finishes and the kill rate becomes more important.
Ir nge ár hmä dispositivo:
UP200St
Referencia yá 'be̲fi ya nthoni:
Joyce, E.; Phull, S. S.; Lorimer, J. P.; Mason, T. J. (2003): The development and evaluation of ultrasound for the treatment of bacterial suspensions. A study of frequency, power and sonication time on cultured Bacillus species. Ultrason. Sonochem. 10/2003. pp. 315-318.
Barley's Alpha-amylase
Nt'ot'e ultrasónica:
Stimulating or inhibiting the activity of Barley’s Alpha-amylase: The sample (10 g barley seeds) was dispersed in 80 ml of tap water at direct sonication at ultrasonic intensity of 20, 60 and 100% amplitude, cyle of 50% and with additional agitation. The sonotrode was immersed about 9 mm into the solution. The solution was processed at constant temperature of 30C° for 5, 10 and 15 min.
Ir nge ár hmä dispositivo:
UP200S, amplitudes: 20, 60 and 100%; cyle of 50%; Sonotrode S3, 30C°.
Referencia yá 'be̲fi ya nthoni:
Yaldagard et al. (2008): Effect of Ultrasonic Power on the Activity of Barley’s Alpha-amylase from Post-sowing Treat of Seeds
Stimulating or inhibiting the activity of Barley’s Alpha-amylase: The sample (10 g barley seeds) was dispersed in 80 ml of tap water at direct sonication at ultrasonic intensity of 20, 60 and 100% amplitude, cyle of 50% and with additional agitation. The sonotrode was immersed about 9 mm into the solution. The solution was processed at constant temperature of 30C° for 5, 10 and 15 min.
Ir nge ár hmä dispositivo:
UP200S, amplitudes: 20, 60 and 100%; cyle of 50%; Sonotrode S3, 30C°.
Referencia yá 'be̲fi ya nthoni:
Yaldagard et al. (2008): Effect of Ultrasonic Power on the Activity of Barley’s Alpha-amylase from Post-sowing Treat of Seeds
Barnacle nauplii
Nt'ot'e ultrasónica:
Cell disruption/ killing of mesozooplankton: by sonication under following conditions of four flow rates (200, 400, 520 and 800 lh-1) and four amplitudes (25, 50, 75 and 100 %) a killing rate between 61 and 97 % has been achieved.
Ir nge ár hmä dispositivo:
UIP2000hd
Referencia yá 'be̲fi ya nthoni:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Ultrasound and Hydrogen Peroxide As Ballast Water Treatments – Experiments with Mesozooplankton In Low Saline- Brackish Water.
Cell disruption/ killing of mesozooplankton: by sonication under following conditions of four flow rates (200, 400, 520 and 800 lh-1) and four amplitudes (25, 50, 75 and 100 %) a killing rate between 61 and 97 % has been achieved.
Ir nge ár hmä dispositivo:
UIP2000hd
Referencia yá 'be̲fi ya nthoni:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Ultrasound and Hydrogen Peroxide As Ballast Water Treatments – Experiments with Mesozooplankton In Low Saline- Brackish Water.
Bifidobacteria strains: B. breve ATCC 15700, B. animalis subsp. Lactis (BB-12), B. longum (BB-46)
Nt'ot'e ultrasónica:
Bacteria cell destruction and release of ß-galactosidase from Bifidobacteria strains: B. breve ATCC 15700, B. animalis subsp. Lactis (BB-12), B. longum (BB-46): 100 mL pasteurized milk blended with bacteria culture was applied with ultrasound. Cavitation causes the destruction of the bacterial cells and at the same time, release of ß-galactosidase.
Ir nge ár hmä dispositivo:
UIP1000hd: amplitude: 10%, power: 80W, amplitude: 100%, power: 200W; Sonotrode BS2d34; 15-30 min.
Referencia yá 'be̲fi ya nthoni:
Hung et al. (2009): Ultrasound Aided Yogurt Fermentation with Probiotics.
Bacteria cell destruction and release of ß-galactosidase from Bifidobacteria strains: B. breve ATCC 15700, B. animalis subsp. Lactis (BB-12), B. longum (BB-46): 100 mL pasteurized milk blended with bacteria culture was applied with ultrasound. Cavitation causes the destruction of the bacterial cells and at the same time, release of ß-galactosidase.
Ir nge ár hmä dispositivo:
UIP1000hd: amplitude: 10%, power: 80W, amplitude: 100%, power: 200W; Sonotrode BS2d34; 15-30 min.
Referencia yá 'be̲fi ya nthoni:
Hung et al. (2009): Ultrasound Aided Yogurt Fermentation with Probiotics.
BL21(DE3) pAtHNL cells (Arabidopsis thaliana cells)
Nt'ot'e ultrasónica:
Cell disruption: 15 g BL21-(DE3)_pAtHNL cells were slowly suspended in 50 mM potassium phosphate buffer (pH 7.5) at 0 degC.
Ir nge ár hmä dispositivo:
UP200S + sonotrode S14D: at 70W/cm2 (4 x 5 min), cooled in an ice bath
Referencia yá 'be̲fi ya nthoni:
Okrob, D. et al (2009): Hydroxynitrile Lyase from Arabidopsis thaliana: Identification of Reaction Parameters for Enantiopure Cyanohydrin Synthesis by Pure and Immobilized Catalyst. Adv. Synth. Catal. 2011, 353, 2399 – 2408.
Cell disruption: 15 g BL21-(DE3)_pAtHNL cells were slowly suspended in 50 mM potassium phosphate buffer (pH 7.5) at 0 degC.
Ir nge ár hmä dispositivo:
UP200S + sonotrode S14D: at 70W/cm2 (4 x 5 min), cooled in an ice bath
Referencia yá 'be̲fi ya nthoni:
Okrob, D. et al (2009): Hydroxynitrile Lyase from Arabidopsis thaliana: Identification of Reaction Parameters for Enantiopure Cyanohydrin Synthesis by Pure and Immobilized Catalyst. Adv. Synth. Catal. 2011, 353, 2399 – 2408.
Blood cells (red and white)
Boldine from Boldo leaves (Peumus boldus Molina)
Nt'ot'e ultrasónica:
'Nar compuesto activo mahyoni ja ar boldo ge ar boldina ((S)-2,9-dihidroxi-1,10-dimetoxiaporfina), ne 'nehe ar catequina ((2S,3R)-2-(3,4-dihidroxi-fenil)-3,4-dihidro-1(2H)-benzopiran-3,5,7-triol) ge ya ndu'mi componentes fracción alcaloide ne flavonoide ja ya hojas boldo.) Ar boldina ge 'nar xí nze̲di agente antioxidante sufre ngi peroxidativo mediado ya radicales libres ne da mats'i nu'u komongu 'nar nt'ot'e xi hño eliminador radicales ar hidroxilo.
Ar nt'ot'e extracción: Pa 'nar nt'ot'e ar extracción típico, ar extrajeron muestras hojas boldo ko 1 litro ar dehe destilada presión atmosférica utilizando ar ultrasonido UIP1000hd jar modo lotes ne ar flujo continuo. Ar pa extracción oscila entre 10 ne 40 min., ko 'nar intensidad ultrasónica 10 jar 23 W yá cm2y un rango de temperatura de 10 a 70 °C. Ya mpädi mäs xi resultados ar obtuvieron jar nuya ya nkohi: intensidad ultrasónica 23 W yá cm2 durante 40 min. a una temperatura de 36°C
Resultados: Ya resultados ar análisis muestran da sonicación mextha nts'edi mejora ar liberación ar analito ar he̲'mi matriz vegetal ar boldo da tasas significativamente mpädi mäs xi jar comparación ko ár nt'ot'e convencional: ar liberó xkagentho ar rendimiento ya sonicación jar 30 ar min., mente ke ar pa extracción convencional bí 2 h.
Chemat (2013) ne yá colaboradores xi demostrado da extracción asistida ya ultrasonidos mejora dätä nt'ot'e extracción ar planta ya pa reduce ar pa extracción ja 'nar dätä concentración ya extractos (xkagentho yá 'bede ya disolvente ne) hñei vegetal). Ar análisis reveló ke ya nkohi optimizadas mi: nts'edi sonicación 23 W yá cm2 ko UIP1000hd Nxoge 40 min. ne 'nar mpat'i 36°C. Ya parámetros optimizados ar extracción ya ultrasonidos proporcionan 'nar mäs xi hño extracción jar comparación ko 'nar maceración convencional jar ngäts'i ar pa proceso (30 ar min. en lugar de 120 min.), dätä rendimiento, dätä dätä nt'ot'e energética, dätä limpieza, Ts'ut'ubi Ntsuni ne xi hño hño ar producto.
Ir nge ár hmä dispositivo:
UIP1000hd sonotrodo BS2d34 ne célula flujo
Referencia yá 'be̲fi ya nthoni:
Petigny, L.; Périno-Issartier, S.; Wajsman, J.; Chemat, F. (2013): Extracción asistida ya ultrasonidos discontinuos ne continuos ar hojas boldo (Peumus boldus mol.). Revista ja ya Ciencia Molecular 14, 2013. 5750-5764.
'Nar compuesto activo mahyoni ja ar boldo ge ar boldina ((S)-2,9-dihidroxi-1,10-dimetoxiaporfina), ne 'nehe ar catequina ((2S,3R)-2-(3,4-dihidroxi-fenil)-3,4-dihidro-1(2H)-benzopiran-3,5,7-triol) ge ya ndu'mi componentes fracción alcaloide ne flavonoide ja ya hojas boldo.) Ar boldina ge 'nar xí nze̲di agente antioxidante sufre ngi peroxidativo mediado ya radicales libres ne da mats'i nu'u komongu 'nar nt'ot'e xi hño eliminador radicales ar hidroxilo.
Ar nt'ot'e extracción: Pa 'nar nt'ot'e ar extracción típico, ar extrajeron muestras hojas boldo ko 1 litro ar dehe destilada presión atmosférica utilizando ar ultrasonido UIP1000hd jar modo lotes ne ar flujo continuo. Ar pa extracción oscila entre 10 ne 40 min., ko 'nar intensidad ultrasónica 10 jar 23 W yá cm2y un rango de temperatura de 10 a 70 °C. Ya mpädi mäs xi resultados ar obtuvieron jar nuya ya nkohi: intensidad ultrasónica 23 W yá cm2 durante 40 min. a una temperatura de 36°C
Resultados: Ya resultados ar análisis muestran da sonicación mextha nts'edi mejora ar liberación ar analito ar he̲'mi matriz vegetal ar boldo da tasas significativamente mpädi mäs xi jar comparación ko ár nt'ot'e convencional: ar liberó xkagentho ar rendimiento ya sonicación jar 30 ar min., mente ke ar pa extracción convencional bí 2 h.
Chemat (2013) ne yá colaboradores xi demostrado da extracción asistida ya ultrasonidos mejora dätä nt'ot'e extracción ar planta ya pa reduce ar pa extracción ja 'nar dätä concentración ya extractos (xkagentho yá 'bede ya disolvente ne) hñei vegetal). Ar análisis reveló ke ya nkohi optimizadas mi: nts'edi sonicación 23 W yá cm2 ko UIP1000hd Nxoge 40 min. ne 'nar mpat'i 36°C. Ya parámetros optimizados ar extracción ya ultrasonidos proporcionan 'nar mäs xi hño extracción jar comparación ko 'nar maceración convencional jar ngäts'i ar pa proceso (30 ar min. en lugar de 120 min.), dätä rendimiento, dätä dätä nt'ot'e energética, dätä limpieza, Ts'ut'ubi Ntsuni ne xi hño hño ar producto.
Ir nge ár hmä dispositivo:
UIP1000hd sonotrodo BS2d34 ne célula flujo
Referencia yá 'be̲fi ya nthoni:
Petigny, L.; Périno-Issartier, S.; Wajsman, J.; Chemat, F. (2013): Extracción asistida ya ultrasonidos discontinuos ne continuos ar hojas boldo (Peumus boldus mol.). Revista ja ya Ciencia Molecular 14, 2013. 5750-5764.
Bovine serum albumin (BSA)
Nt'ot'e ultrasónica:
Microencapsulation in poly(lactic-co-glycolic acid) in 40 sec.
Ir nge ár hmä dispositivo:
Dmini
Referencia yá 'be̲fi ya nthoni:
Freitas et al. (2005): Flow-through ultrasonic emulsification combined with static micromixing for aseptic production of microspheres by solvent extraction.
Microencapsulation in poly(lactic-co-glycolic acid) in 40 sec.
Ir nge ár hmä dispositivo:
Dmini
Referencia yá 'be̲fi ya nthoni:
Freitas et al. (2005): Flow-through ultrasonic emulsification combined with static micromixing for aseptic production of microspheres by solvent extraction.
Brain stem + adrenal gland
Nt'ot'e ultrasónica:
Dispersion and nucleotid analysis; sample size: 10 mg samples in 10 ml fluid.
Ir nge ár hmä dispositivo:
UP50H
Dispersion and nucleotid analysis; sample size: 10 mg samples in 10 ml fluid.
Ir nge ár hmä dispositivo:
UP50H
Candida albicans
Carbohydrates, polysaccharides and other functional compounds
Nt'ot'e ultrasónica:
Extraction of carbohydrates, polysaccharides and other functional compounds
Ir nge ár hmä dispositivo:
UP200St
Extraction of carbohydrates, polysaccharides and other functional compounds
Ir nge ár hmä dispositivo:
UP200St
Carnosic acid from rosemary
Nt'ot'e ultrasónica:
Extraction of carnosic acid, an active compound, from rosemary.
Ir nge ár hmä dispositivo:
UP400S
Extraction of carnosic acid, an active compound, from rosemary.
Ir nge ár hmä dispositivo:
UP400S
Capsaicinoides
Nt'ot'e ultrasónica:
Extracción capsaicinoides (capsaicina, nordihidrocapsaicina) ar chiles: Capsaicinoides ar Capsicum frutescens Ya pimientos ar obtuvieron ir nge ya extracción ultrasónica jar nuya ya nkohi: disolvente: etanol 95% (v/v), nthe disolvente yá masa ya 10 ml yá g, pa extracción ya sonicación 40 min, mpat'i extracción 45°c.ndunthe Rendimiento ar extractante: 85% ja ya capsaicinoides
Ir nge ár hmä dispositivo:
UP400S
Extracción capsaicinoides (capsaicina, nordihidrocapsaicina) ar chiles: Capsaicinoides ar Capsicum frutescens Ya pimientos ar obtuvieron ir nge ya extracción ultrasónica jar nuya ya nkohi: disolvente: etanol 95% (v/v), nthe disolvente yá masa ya 10 ml yá g, pa extracción ya sonicación 40 min, mpat'i extracción 45°c.ndunthe Rendimiento ar extractante: 85% ja ya capsaicinoides
Ir nge ár hmä dispositivo:
UP400S
Carotenoids, Beta-Carotenoids
cDNA
Nt'ot'e ultrasónica:
Poly-A RNA was purified with the Dynabeads mRNA purification kit (Invitrogen) following the manufacturer’s instructions and was treated for 30 min at 37°C with TURBO DNase (Ambion; 0.2 units/1 μg of RNA). First- and second-strand synthesis followed the manufacturer’s protocol. About 500ng of double-stranded cDNA was fragmented by sonication with Hielscher’s UTR200. DNA was parceled in 2% high-resolution agarose gel and 230–270 bp fragments were cut.
Ir nge ár hmä dispositivo:
UTR200 o TD_CupHorn
Referencia yá 'be̲fi ya nthoni:
Delft, J. van; Gaj, St.; Lienhard,M.; Albrecht, M. W.; Kirpiy, A.; Brauers, K.; Claessen, S.; Lizarraga, D.; Lehrach, H.; Herwig, R.; Kleinjans, J. (2012): RNA-Seq Provides New Insights in the Transcriptome Responses Induced by the Carcinogen Benzo[a]pyrene. Toxicological Sciences 130/2, 2012. 427–439.
Poly-A RNA was purified with the Dynabeads mRNA purification kit (Invitrogen) following the manufacturer’s instructions and was treated for 30 min at 37°C with TURBO DNase (Ambion; 0.2 units/1 μg of RNA). First- and second-strand synthesis followed the manufacturer’s protocol. About 500ng of double-stranded cDNA was fragmented by sonication with Hielscher’s UTR200. DNA was parceled in 2% high-resolution agarose gel and 230–270 bp fragments were cut.
Ir nge ár hmä dispositivo:
UTR200 o TD_CupHorn
Referencia yá 'be̲fi ya nthoni:
Delft, J. van; Gaj, St.; Lienhard,M.; Albrecht, M. W.; Kirpiy, A.; Brauers, K.; Claessen, S.; Lizarraga, D.; Lehrach, H.; Herwig, R.; Kleinjans, J. (2012): RNA-Seq Provides New Insights in the Transcriptome Responses Induced by the Carcinogen Benzo[a]pyrene. Toxicological Sciences 130/2, 2012. 427–439.
Caryophanon latum
Nt'ot'e ultrasónica:
Extraction of glucosamine, muramic acid, alanine, glutamic acid and lysine from caryophanon datum.
Ir nge ár hmä dispositivo:
UP100H
Extraction of glucosamine, muramic acid, alanine, glutamic acid and lysine from caryophanon datum.
Ir nge ár hmä dispositivo:
UP100H
celulosa
Nt'ot'e ultrasónica:
Homogenization/ preparation of isotopically homogeneous cellulose.
Ir nge ár hmä dispositivo:
UP200S; in an ice bath
Referencia yá 'be̲fi ya nthoni:
Laumer et al. (2009): A novel approach for the homogenization of cellulose to use microamounts for stable isotope analyses.
Homogenization/ preparation of isotopically homogeneous cellulose.
Ir nge ár hmä dispositivo:
UP200S; in an ice bath
Referencia yá 'be̲fi ya nthoni:
Laumer et al. (2009): A novel approach for the homogenization of cellulose to use microamounts for stable isotope analyses.
Cellulose nanocrystals (CNC) prepared from eucalyptus cellulose CNCs
Nt'ot'e ultrasónica:
Cellulose nanocrystals (CNC) prepared from eucalyptus cellulose CNCs were modified by the reaction with methyl adipoyl chloride, CNCm, or with a mixture of acetic and sulfuric acid, CNCa. Therefore, freeze-dried CNCs, CNCm and CNCa were redispersed in pure solvents (EA, THF or DMF) at 0.1 wt%, bymagnetic stirring overnight at (24 ± 1) C, followed by 20 min in a sonication bath using UP100H Hielscher Ultrasonics (Germany), equipped with a 130 W/cm2 sonotrode, at 24 ± 1 degC. After that, CAB was added to the CNC dispersion, so that the final polymer concentration was 0.9 wt%.
Ir nge ár hmä dispositivo:
UP100H; at 24 degC for 20 min.
Referencia yá 'be̲fi ya nthoni:
Blachechen, L. S. et al (2013): Interplay of colloidal stability of cellulose nanocrystals and their dispersibility in cellulose acetate butyrate matrix. Cellulose 2013.
Cellulose nanocrystals (CNC) prepared from eucalyptus cellulose CNCs were modified by the reaction with methyl adipoyl chloride, CNCm, or with a mixture of acetic and sulfuric acid, CNCa. Therefore, freeze-dried CNCs, CNCm and CNCa were redispersed in pure solvents (EA, THF or DMF) at 0.1 wt%, bymagnetic stirring overnight at (24 ± 1) C, followed by 20 min in a sonication bath using UP100H Hielscher Ultrasonics (Germany), equipped with a 130 W/cm2 sonotrode, at 24 ± 1 degC. After that, CAB was added to the CNC dispersion, so that the final polymer concentration was 0.9 wt%.
Ir nge ár hmä dispositivo:
UP100H; at 24 degC for 20 min.
Referencia yá 'be̲fi ya nthoni:
Blachechen, L. S. et al (2013): Interplay of colloidal stability of cellulose nanocrystals and their dispersibility in cellulose acetate butyrate matrix. Cellulose 2013.
Cellulose from sugarcane bagasse
Nt'ot'e ultrasónica:
Extraction of cellulose from sugarcane bagasse
Ir nge ár hmä dispositivo:
UP200St
Extraction of cellulose from sugarcane bagasse
Ir nge ár hmä dispositivo:
UP200St
Ensayo ChIP
Nt'ot'e ultrasónica:
Ultrasonication is used for cell lysis to release the chromatin. Mild (pulsed) sonication is used to fragment the chromatin. Furthermore, ultrasound accelerates the rate of antibody binding to target proteins and reduces thereby the immunoprecipitation time.
Ir nge ár hmä dispositivo:
UP100H
Referencia yá 'be̲fi ya nthoni:
Basselet, P. et al. (2008): Sample processing for DNA chip array-based analysis of enterohemorrhagic Escherichia coli (EHEC).
Lauri, A. (2005): Molecular analysis of petal development by X-ChIP and two-hybrid technology. Dissertation University of Cologne 2005.
Ultrasonication is used for cell lysis to release the chromatin. Mild (pulsed) sonication is used to fragment the chromatin. Furthermore, ultrasound accelerates the rate of antibody binding to target proteins and reduces thereby the immunoprecipitation time.
Ir nge ár hmä dispositivo:
UP100H
Referencia yá 'be̲fi ya nthoni:
Basselet, P. et al. (2008): Sample processing for DNA chip array-based analysis of enterohemorrhagic Escherichia coli (EHEC).
Lauri, A. (2005): Molecular analysis of petal development by X-ChIP and two-hybrid technology. Dissertation University of Cologne 2005.
cromatina
Nt'ot'e ultrasónica:
Shearing of chromatin
Ir nge ár hmä dispositivo:
UP400S; at 30% amplitude and 0.5 cycle; on ice.
Referencia yá 'be̲fi ya nthoni:
Oh et al. (2003): Acetyl-CoA Carboxylase Gene Is Regulated by Sterol Regulatory Element-binding Protein-1 in Liver.
Shearing of chromatin
Ir nge ár hmä dispositivo:
UP400S; at 30% amplitude and 0.5 cycle; on ice.
Referencia yá 'be̲fi ya nthoni:
Oh et al. (2003): Acetyl-CoA Carboxylase Gene Is Regulated by Sterol Regulatory Element-binding Protein-1 in Liver.
Chromatin extraction
Nt'ot'e ultrasónica:
The lysate of MEL DS19 cells was sonicated with 10 rounds of 20 s each at 70% of maximum output using the Hielscher 200W ultrasonic processor UP200H
Ir nge ár hmä dispositivo:
UP200H; 70% of maximum output; pulse mode: 10 cycles of 20 sec. each.
Referencia yá 'be̲fi ya nthoni:
Kang, H. Ch. et al (2010): PIAS1 regulates CP2c localization and active promoter complex formation in erythroid cell-specific a-globin expression. Nucleic Acids Res. 38/ 16, 2010. pp. 5456–5471.
The lysate of MEL DS19 cells was sonicated with 10 rounds of 20 s each at 70% of maximum output using the Hielscher 200W ultrasonic processor UP200H
Ir nge ár hmä dispositivo:
UP200H; 70% of maximum output; pulse mode: 10 cycles of 20 sec. each.
Referencia yá 'be̲fi ya nthoni:
Kang, H. Ch. et al (2010): PIAS1 regulates CP2c localization and active promoter complex formation in erythroid cell-specific a-globin expression. Nucleic Acids Res. 38/ 16, 2010. pp. 5456–5471.
cromatografía
Nt'ot'e ultrasónica:
Sonication of the adsorbant in the solvent eliminates agglomerates within a few seconds and prepares a uniform, easily packed column. Relevant for adsorbent preparation (e.g. silica gel) prior to column chromatography.
Ir nge ár hmä dispositivo:
UP400S
Sonication of the adsorbant in the solvent eliminates agglomerates within a few seconds and prepares a uniform, easily packed column. Relevant for adsorbent preparation (e.g. silica gel) prior to column chromatography.
Ir nge ár hmä dispositivo:
UP400S
Cladocerans
Nt'ot'e ultrasónica:
Cell disruption of mesozooplankton
Ir nge ár hmä dispositivo:
UP2000hd with flow cell
Referencia yá 'be̲fi ya nthoni:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Ultrasound and Hydrogen Peroxide As Ballast Water Treatments – Experiments with Mesozooplankton In Low Saline- Brackish Water.
Cell disruption of mesozooplankton
Ir nge ár hmä dispositivo:
UP2000hd with flow cell
Referencia yá 'be̲fi ya nthoni:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Ultrasound and Hydrogen Peroxide As Ballast Water Treatments – Experiments with Mesozooplankton In Low Saline- Brackish Water.
Copepod adults and copepodites
Nt'ot'e ultrasónica:
Cell disruption of mesozooplankton: by sonication under following conditions of four flow rates (200, 400, 520 and 800 lh-1) and four amplitudes (25, 50, 75 and 100%) a killing rate between 87 and 99% has been achieved.
Ir nge ár hmä dispositivo:
UIP2000hd with flow cell
Referencia yá 'be̲fi ya nthoni:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Ultrasound and Hydrogen Peroxide As Ballast Water Treatments – Experiments with Mesozooplankton In Low Saline- Brackish Water.
Cell disruption of mesozooplankton: by sonication under following conditions of four flow rates (200, 400, 520 and 800 lh-1) and four amplitudes (25, 50, 75 and 100%) a killing rate between 87 and 99% has been achieved.
Ir nge ár hmä dispositivo:
UIP2000hd with flow cell
Referencia yá 'be̲fi ya nthoni:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Ultrasound and Hydrogen Peroxide As Ballast Water Treatments – Experiments with Mesozooplankton In Low Saline- Brackish Water.
Copepod nauplii
Nt'ot'e ultrasónica:
Cell disruption of mesozooplankton: by sonication under following conditions of four flow rates (200, 400, 520 and 800 lh-1) and four amplitudes (25, 50, 75 and 100%) a killing rate between 87 and 99% has been achieved.
Ir nge ár hmä dispositivo:
UIP2000hd with flow cell
Referencia yá 'be̲fi ya nthoni:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Ultrasound and Hydrogen Peroxide As Ballast Water Treatments – Experiments with Mesozooplankton In Low Saline- Brackish Water.
Cell disruption of mesozooplankton: by sonication under following conditions of four flow rates (200, 400, 520 and 800 lh-1) and four amplitudes (25, 50, 75 and 100%) a killing rate between 87 and 99% has been achieved.
Ir nge ár hmä dispositivo:
UIP2000hd with flow cell
Referencia yá 'be̲fi ya nthoni:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Ultrasound and Hydrogen Peroxide As Ballast Water Treatments – Experiments with Mesozooplankton In Low Saline- Brackish Water.
COS7-cells
Nt'ot'e ultrasónica:
Lysis in 400 μl buffer
Ir nge ár hmä dispositivo:
UP200S; 7 cycles
Referencia yá 'be̲fi ya nthoni:
Zaim (2005): Analyse von Nesprin-2 Defizienten Mäusen.
Lysis in 400 μl buffer
Ir nge ár hmä dispositivo:
UP200S; 7 cycles
Referencia yá 'be̲fi ya nthoni:
Zaim (2005): Analyse von Nesprin-2 Defizienten Mäusen.
Cryptosporidium parvaum
Nt'ot'e ultrasónica:
Ultrasonic inactivation of Cryptosporidium parvaum (protozoa) in water.
Ir nge ár hmä dispositivo:
UP400S
Referencia yá 'be̲fi ya nthoni:
Tsukamoto, I.; Yim, B.; Stavarache, C. E.; Furuta, M.; Hashiba, K.; Maeda, Y. (2004): Inactivation of Saccharomyces cerevisiae by ultrasonic irradiation. Ultrasonics Sonochemistry 11/2004. pp. 61–65.
Ultrasonic inactivation of Cryptosporidium parvaum (protozoa) in water.
Ir nge ár hmä dispositivo:
UP400S
Referencia yá 'be̲fi ya nthoni:
Tsukamoto, I.; Yim, B.; Stavarache, C. E.; Furuta, M.; Hashiba, K.; Maeda, Y. (2004): Inactivation of Saccharomyces cerevisiae by ultrasonic irradiation. Ultrasonics Sonochemistry 11/2004. pp. 61–65.
Cubosomes
Nt'ot'e ultrasónica:
Cubosomes – either empty or doped with fluorophore molecules – were prepared by dispersing the appropriate amount of monoolein in a solution of Pluronic F108 using the ultrasonicator UP100H. To obtain fluorescent cubosomes, the fluorophore was dispersed in the melted monoolein by gentle sonification before dispersion in Pluronic F108. The same procedure was followed when the fluorescent cubosomes were also loaded with quercetin.
Sample: sample size: 4 mL – approx. 96.4 wt% of water, 3.3 wt % of monoolein, 0.3 wt% of Pluronic F108. The percentage of the fluorophore was 2.5 × 10−3 and 2.8 × 10−3 wt%. Amount of added quercetin: 6.4 × 10−6 wt%.
Sonicación: UP100H, amplitude 90%, pulse cycle 0.9, for 10 min.
Ir nge ár hmä dispositivo:
UP100H
Referencia yá 'be̲fi ya nthoni:
Murgia, S.; Bonacchi,S.; Falchi, A. M.; Lampis, S.; Lippolis, V.; Meli, V.; Monduzzi, M.; Prodi, L.; Schmidt, J.; Talmon, Y.; Caltagirone, C. (2013): Drug-Loaded Fluorescent Cubosomes: Versatile Nanoparticles for Potential Theranostic Applications. Langmuir 29, 2013. 6673-6679.
Cubosomes – either empty or doped with fluorophore molecules – were prepared by dispersing the appropriate amount of monoolein in a solution of Pluronic F108 using the ultrasonicator UP100H. To obtain fluorescent cubosomes, the fluorophore was dispersed in the melted monoolein by gentle sonification before dispersion in Pluronic F108. The same procedure was followed when the fluorescent cubosomes were also loaded with quercetin.
Sample: sample size: 4 mL – approx. 96.4 wt% of water, 3.3 wt % of monoolein, 0.3 wt% of Pluronic F108. The percentage of the fluorophore was 2.5 × 10−3 and 2.8 × 10−3 wt%. Amount of added quercetin: 6.4 × 10−6 wt%.
Sonicación: UP100H, amplitude 90%, pulse cycle 0.9, for 10 min.
Ir nge ár hmä dispositivo:
UP100H
Referencia yá 'be̲fi ya nthoni:
Murgia, S.; Bonacchi,S.; Falchi, A. M.; Lampis, S.; Lippolis, V.; Meli, V.; Monduzzi, M.; Prodi, L.; Schmidt, J.; Talmon, Y.; Caltagirone, C. (2013): Drug-Loaded Fluorescent Cubosomes: Versatile Nanoparticles for Potential Theranostic Applications. Langmuir 29, 2013. 6673-6679.
Dekkera bruxellensis
Nt'ot'e ultrasónica:
Ultrasonic inactivation of Dekkera bruxellensis in water
Ir nge ár hmä dispositivo:
UIP1500hd
Referencia yá 'be̲fi ya nthoni:
Borthwick, K. A. J.; Coakley, W. T.; McDonnell, M. B.; Nowotny, H.; Benes, E.; Grfschl, .M (2005): Development of a novel compact sonicator for cell disruption. J. Microbio. Meths. 60/2005. pp. 207–216. / Lörincz, A. (2004): Ultrasonic cellular disruption of yeast in water-based suspensions. Biosys. Eng. 89/ 2004. pp. 297–308. / Tsukamoto, I.; Yim, B.; Stavarache, C. E.; Furuta, M.; Hashiba, K.; Maeda, Y. (2004): Inactivation of Saccharomyces cerevisiae by ultrasonic irradiation. Ultrason. Sonochem. 11/2004. pp. 61–65.
Ultrasonic inactivation of Dekkera bruxellensis in water
Ir nge ár hmä dispositivo:
UIP1500hd
Referencia yá 'be̲fi ya nthoni:
Borthwick, K. A. J.; Coakley, W. T.; McDonnell, M. B.; Nowotny, H.; Benes, E.; Grfschl, .M (2005): Development of a novel compact sonicator for cell disruption. J. Microbio. Meths. 60/2005. pp. 207–216. / Lörincz, A. (2004): Ultrasonic cellular disruption of yeast in water-based suspensions. Biosys. Eng. 89/ 2004. pp. 297–308. / Tsukamoto, I.; Yim, B.; Stavarache, C. E.; Furuta, M.; Hashiba, K.; Maeda, Y. (2004): Inactivation of Saccharomyces cerevisiae by ultrasonic irradiation. Ultrason. Sonochem. 11/2004. pp. 61–65.
Dekkera/ Brettanomyces bruxellensis
Nt'ot'e ultrasónica:
Inactivation in 90-120 sec.
Ir nge ár hmä dispositivo:
UIP1500hd
Referencia yá 'be̲fi ya nthoni:
see above
Inactivation in 90-120 sec.
Ir nge ár hmä dispositivo:
UIP1500hd
Referencia yá 'be̲fi ya nthoni:
see above
ADN
Nt'ot'e ultrasónica:
DNA fragmentation: 2 min. sonication of 100μL with UP100H or 4 min. sonication of 100μL with UTR200.
Ir nge ár hmä dispositivo:
UP100H, UTR200 o VialTweeter
Referencia yá 'be̲fi ya nthoni:
Larguinho M. et al. (2010): Development of a fast and efficient ultrasonic-based strategy for DNA fragmentation.
DNA fragmentation: 2 min. sonication of 100μL with UP100H or 4 min. sonication of 100μL with UTR200.
Ir nge ár hmä dispositivo:
UP100H, UTR200 o VialTweeter
Referencia yá 'be̲fi ya nthoni:
Larguinho M. et al. (2010): Development of a fast and efficient ultrasonic-based strategy for DNA fragmentation.
Drosophila melanogaster S2 cells
Nt'ot'e ultrasónica:
Extraction of cellular proteins from Drosophila melanogaster S2 cells: Frozen S2 cells (1.56108) were lysed in 1 mL ice-cold homogenization buffer (100 mM Tris-HCl pH 7.5, 1% SDS) and left on ice for 10 min. Cell lysis was completed by ultrasonication on ice (6615 bursts, 0.5 s pulse; 75% intensity) with a Hielscher UP200S ultrasonic processor.
Ir nge ár hmä dispositivo:
UP200S (200W), 75% intensitypulse mode: 6615 bursts, 0.5 s pulse.
Referencia yá 'be̲fi ya nthoni:
Schwientek, T. et al. (2007): A serial lectin approach to the mucin-type O-glycoproteome of Drosophila melanogaster S2 cells. Proteomics 7, 2007. pp. 3264-3277.
Extraction of cellular proteins from Drosophila melanogaster S2 cells: Frozen S2 cells (1.56108) were lysed in 1 mL ice-cold homogenization buffer (100 mM Tris-HCl pH 7.5, 1% SDS) and left on ice for 10 min. Cell lysis was completed by ultrasonication on ice (6615 bursts, 0.5 s pulse; 75% intensity) with a Hielscher UP200S ultrasonic processor.
Ir nge ár hmä dispositivo:
UP200S (200W), 75% intensitypulse mode: 6615 bursts, 0.5 s pulse.
Referencia yá 'be̲fi ya nthoni:
Schwientek, T. et al. (2007): A serial lectin approach to the mucin-type O-glycoproteome of Drosophila melanogaster S2 cells. Proteomics 7, 2007. pp. 3264-3277.
E. coli derivates
Nt'ot'e ultrasónica:
Cell disruption of E. coli derivates: The frozen pellets corresponding to the sample volume of Vculture = 4/OD mL were resuspended in 580 μL 10 mM potassium phosphate buffer pH 7, 1 mM EDTA. 20 μL of lysozyme (concentration of 1 g L-1) was added and the cells suspension was incubated on ice for about 30 min. Afterwards cells were disrupted by continuous ultrasonication with an ultrasonicator (UP 200S Ultraschallprozessor, Dr. Hielscher GmbH, Teltow) on ice, at an amplitude of 50 % for 20 sec. The soluble and insoluble cell fractions were separated by centrifugation at 13000 rpm for 20 min. The insoluble proteins were washed twice with the 10 mM potassium phosphate buffer pH 7, 1 mM EDTA and stored at –20°C.
Ir nge ár hmä dispositivo:
UP200S with 50% amplitude
Referencia yá 'be̲fi ya nthoni:
HA (2005): Optimisation of active recombinant protein production, exploring the impact of small heat-shock proteins of Escherichia coli, IbpA and IbpB, on in vivo reactivation of inclusion bodies.
Cell disruption of E. coli derivates: The frozen pellets corresponding to the sample volume of Vculture = 4/OD mL were resuspended in 580 μL 10 mM potassium phosphate buffer pH 7, 1 mM EDTA. 20 μL of lysozyme (concentration of 1 g L-1) was added and the cells suspension was incubated on ice for about 30 min. Afterwards cells were disrupted by continuous ultrasonication with an ultrasonicator (UP 200S Ultraschallprozessor, Dr. Hielscher GmbH, Teltow) on ice, at an amplitude of 50 % for 20 sec. The soluble and insoluble cell fractions were separated by centrifugation at 13000 rpm for 20 min. The insoluble proteins were washed twice with the 10 mM potassium phosphate buffer pH 7, 1 mM EDTA and stored at –20°C.
Ir nge ár hmä dispositivo:
UP200S with 50% amplitude
Referencia yá 'be̲fi ya nthoni:
HA (2005): Optimisation of active recombinant protein production, exploring the impact of small heat-shock proteins of Escherichia coli, IbpA and IbpB, on in vivo reactivation of inclusion bodies.
Echinococcus granulosus Antigen
Nt'ot'e ultrasónica:
Cell lysis and disintegration: The homogenized sample of protein soluble of mature E. granulosus, prepared by freeze-thawing in liquid nitrogen and 42◦C, has been sonicated at 110V, 170W for 3×15 sec on ice. After that, the sample was centrifugated for 15min at 10000g. Protein concentaration was measured by Bradford method and stored at -20◦C.
Ir nge ár hmä dispositivo:
UP200S; 170W, cooling on ice; 3 x 15 sec.
Referencia yá 'be̲fi ya nthoni:
Tabar et al. (2010): Serodiagnosis of Sheep Hydatidosis with Hydatid Fluid, Protoscolex and Whole Body of Echinococcus granulosus Antigen.
Cell lysis and disintegration: The homogenized sample of protein soluble of mature E. granulosus, prepared by freeze-thawing in liquid nitrogen and 42◦C, has been sonicated at 110V, 170W for 3×15 sec on ice. After that, the sample was centrifugated for 15min at 10000g. Protein concentaration was measured by Bradford method and stored at -20◦C.
Ir nge ár hmä dispositivo:
UP200S; 170W, cooling on ice; 3 x 15 sec.
Referencia yá 'be̲fi ya nthoni:
Tabar et al. (2010): Serodiagnosis of Sheep Hydatidosis with Hydatid Fluid, Protoscolex and Whole Body of Echinococcus granulosus Antigen.
Escherchia coli Gr-
Nt'ot'e ultrasónica:
Ultrasonic inactivation of Escherchia coli Gr- in milk and juices.
Ir nge ár hmä dispositivo:
UP100H
Referencia yá 'be̲fi ya nthoni:
Zenker, M.; Heinz, V.; Knorr, D. (2003): Application of ultrasound assisted thermal processing for preservation and quality retention of liquid foods. J Food Prot 66/2003. pp. 1642–1649.
Ultrasonic inactivation of Escherchia coli Gr- in milk and juices.
Ir nge ár hmä dispositivo:
UP100H
Referencia yá 'be̲fi ya nthoni:
Zenker, M.; Heinz, V.; Knorr, D. (2003): Application of ultrasound assisted thermal processing for preservation and quality retention of liquid foods. J Food Prot 66/2003. pp. 1642–1649.
Escherchia coli Gr- in saline
Nt'ot'e ultrasónica:
Ultrasonic inactivation of Escherchia coli Gr- in saline.
Ir nge ár hmä dispositivo:
UP100H
Referencia yá 'be̲fi ya nthoni:
Duckhouse, H.; Mason, T. J.; Phull, S. S.; Lorimer, J. P. (2004): The effect of sonication on microbial disinfection using hypochlorite. Ultrason. Sonochem. 11/ 2004. pp. 173–176.
Ultrasonic inactivation of Escherchia coli Gr- in saline.
Ir nge ár hmä dispositivo:
UP100H
Referencia yá 'be̲fi ya nthoni:
Duckhouse, H.; Mason, T. J.; Phull, S. S.; Lorimer, J. P. (2004): The effect of sonication on microbial disinfection using hypochlorite. Ultrason. Sonochem. 11/ 2004. pp. 173–176.
Escherchia coli Gr- in water
Nt'ot'e ultrasónica:
Ultrasonic inactivation of Escherchia coli Gr- in water.
Ir nge ár hmä dispositivo:
UP100H
Referencia yá 'be̲fi ya nthoni:
Furuta, M.; Yamaguchi, M.; Tsukamoto, T.; Yim, B.; Stavarache, C. E.; Hasiba, K.; Maeda, Y. (2004): Inactivation of Escherichia coli by ultrasonic irradiation. Ultrason. Sonochem. 11/2004. pp. 57–60.
Ultrasonic inactivation of Escherchia coli Gr- in water.
Ir nge ár hmä dispositivo:
UP100H
Referencia yá 'be̲fi ya nthoni:
Furuta, M.; Yamaguchi, M.; Tsukamoto, T.; Yim, B.; Stavarache, C. E.; Hasiba, K.; Maeda, Y. (2004): Inactivation of Escherichia coli by ultrasonic irradiation. Ultrason. Sonochem. 11/2004. pp. 57–60.
Glaucoma, optical nerve head
Nt'ot'e ultrasónica:
RNA fragmentation of optical nerve heads (from rat eyes): The optical nerve head (ONH) was frozen on dry ice and stored at 80°C before extraction. RNA was isolated by sonicating the frozen nerve heads in the kit extraction buffer using an MS 0.5 probe (UP50H) and then, after the instructions provided by the Arcturus kit, including DNase treatment to remove any DNA. Purified RNA was quantified.
Ir nge ár hmä dispositivo:
UP50H with probe MS0.5
Referencia yá 'be̲fi ya nthoni:
Johnson et al. (2007): Global Changes in Optic Nerve Head Gene Expression after Exposure to Elevated Intraocular Pressure in a Rat Glaucoma Model.
RNA fragmentation of optical nerve heads (from rat eyes): The optical nerve head (ONH) was frozen on dry ice and stored at 80°C before extraction. RNA was isolated by sonicating the frozen nerve heads in the kit extraction buffer using an MS 0.5 probe (UP50H) and then, after the instructions provided by the Arcturus kit, including DNase treatment to remove any DNA. Purified RNA was quantified.
Ir nge ár hmä dispositivo:
UP50H with probe MS0.5
Referencia yá 'be̲fi ya nthoni:
Johnson et al. (2007): Global Changes in Optic Nerve Head Gene Expression after Exposure to Elevated Intraocular Pressure in a Rat Glaucoma Model.
Glycosaminoglycan chondroitin sulfate (CS)
Nt'ot'e ultrasónica:
Determining CS by Dimethylmethylene Blue Assay
Resuspension of cells: CS pellets in microcentrifuge tubes were resuspended in 0.5 ml of a 0.1mg/ml papain solution in Hank’s balanced salt solution (HBSS) using pulses from an ultrasound horn (UP 100H, Hielscher Ultrasonics GmbH, Germany) at cycle 1 and 100 % amplitude for 3 sec. Thereafter, digestion took place at 60 °C for 24 h.
For Enzyme-linked immunosorbent assay (ELISA), the cells were then suspended in distilled and deionized water at a concentration of 106 cells/ml, and were kept in a freezer at –70°C overnight in order to facilitate cell lysis. The cells were then homogenized by ultrasonication for 40 sec. using the Hielscher ultrasonic tissue homogenizer UP100H.
Ir nge ár hmä dispositivo:
UP100H
Referencia yá 'be̲fi ya nthoni:
Vandrovcova et al. (2011): Influence of Collagen and Chondroitin Sulfate (CS) Coatings on Poly-(Lactide-co-Glycolide) (PLGA) on MG 63 Osteoblast-Like Cells. Physiol. Res. 60; 2011. 797-813.
Determining CS by Dimethylmethylene Blue Assay
Resuspension of cells: CS pellets in microcentrifuge tubes were resuspended in 0.5 ml of a 0.1mg/ml papain solution in Hank’s balanced salt solution (HBSS) using pulses from an ultrasound horn (UP 100H, Hielscher Ultrasonics GmbH, Germany) at cycle 1 and 100 % amplitude for 3 sec. Thereafter, digestion took place at 60 °C for 24 h.
For Enzyme-linked immunosorbent assay (ELISA), the cells were then suspended in distilled and deionized water at a concentration of 106 cells/ml, and were kept in a freezer at –70°C overnight in order to facilitate cell lysis. The cells were then homogenized by ultrasonication for 40 sec. using the Hielscher ultrasonic tissue homogenizer UP100H.
Ir nge ár hmä dispositivo:
UP100H
Referencia yá 'be̲fi ya nthoni:
Vandrovcova et al. (2011): Influence of Collagen and Chondroitin Sulfate (CS) Coatings on Poly-(Lactide-co-Glycolide) (PLGA) on MG 63 Osteoblast-Like Cells. Physiol. Res. 60; 2011. 797-813.
HaCaT cells
Nt'ot'e ultrasónica:
Lysis of HaCaT cells for Chromatin Immunoprecipitation (ChIP): HaCaT cells were fixed with 1% formaldehyde for 10 minutes at 37uC. The fixed cells were lysed in the RIPA buffer and sonicated on ice with an 100W ultrasonic device at amplitude = 1 and duty cycle = 100% in 12 one-minute (12 x 1 min.) pulses.
Ir nge ár hmä dispositivo:
UP100H; for 12 min. on ice,
Referencia yá 'be̲fi ya nthoni:
Zhang et al. (2007): Basonuclin Regulates a Subset of Ribosomal RNA Genes in HaCaT Cells.
Lysis of HaCaT cells for Chromatin Immunoprecipitation (ChIP): HaCaT cells were fixed with 1% formaldehyde for 10 minutes at 37uC. The fixed cells were lysed in the RIPA buffer and sonicated on ice with an 100W ultrasonic device at amplitude = 1 and duty cycle = 100% in 12 one-minute (12 x 1 min.) pulses.
Ir nge ár hmä dispositivo:
UP100H; for 12 min. on ice,
Referencia yá 'be̲fi ya nthoni:
Zhang et al. (2007): Basonuclin Regulates a Subset of Ribosomal RNA Genes in HaCaT Cells.
Heparin: Depolymerization of heparin
Nt'ot'e ultrasónica:
The ultrasonic method enables to produce a low molecular weight heparin (LMWH). Therefore, heparin undergoes a hydrogen peroxide-catalyzed radical depolymerization. The reaction is very fast and takes less than 1 hour, whilst the physicochemical depolymerization process relies on mild reaction conditions, requires no harsh or toxic reagents, and provides a product free of chemical artifacts. Thus, the ultrasonic process is well suited for the large scale production of LMWH, but also analogs produced from natural sulfated polysaccharides.
Ultrasonic Procedure:
For the physicochemical depolymerization of unfractionated heparin by ultrasonic-assisted radical depolymerization, heparin was dissolved in water to a final concentration of 25 mg/mL (5 mL). The reaction mixture was sonicated using a probe-type ultrasonicator UP50H. The ultrasonicator was equipped with a probe (micro tip MS3) with a diameter of 3mm, which provided an amplitude of 180μm. The processor generated mechanical longitudinal vibrations with a frequency of 30 kHz that were produced by electrical excitation. The reaction mixture was maintained at 60°C in a Radleys® reactor and ultrasonic waves were applied as a 0.5sec pulse to prevent mixture heating. A zerotime aliquot (250μl) was removed from the solution prior to reaction launching by simultaneous addition of hydrogen peroxide and application of ultrasonic waves. Hydrogen peroxide was added to obtain a final hydrogen peroxide/heparin (w/w) ratio of 0.15 (3.75 mg/mL).
Ir nge ár hmä dispositivo:
UP50H with sonotrode MS3
Referencia yá 'be̲fi ya nthoni:
Achour, Oussama; Bridiau, Nicolas; Godhbani, Azza; Le Joubioux, Florian; Bordenave Juchereau, Stephanie; Sannier, Fredéric; Piot, Jean-Marie; Fruitier Arnaudin, Ingrid; Maugard, Thierry (2013): Ultrasonic-assisted preparation of a low molecular weight heparin (LMWH) with anticoagulant activity. Carbohydrate Polymers 97; 2013. 684–689.
The ultrasonic method enables to produce a low molecular weight heparin (LMWH). Therefore, heparin undergoes a hydrogen peroxide-catalyzed radical depolymerization. The reaction is very fast and takes less than 1 hour, whilst the physicochemical depolymerization process relies on mild reaction conditions, requires no harsh or toxic reagents, and provides a product free of chemical artifacts. Thus, the ultrasonic process is well suited for the large scale production of LMWH, but also analogs produced from natural sulfated polysaccharides.
Ultrasonic Procedure:
For the physicochemical depolymerization of unfractionated heparin by ultrasonic-assisted radical depolymerization, heparin was dissolved in water to a final concentration of 25 mg/mL (5 mL). The reaction mixture was sonicated using a probe-type ultrasonicator UP50H. The ultrasonicator was equipped with a probe (micro tip MS3) with a diameter of 3mm, which provided an amplitude of 180μm. The processor generated mechanical longitudinal vibrations with a frequency of 30 kHz that were produced by electrical excitation. The reaction mixture was maintained at 60°C in a Radleys® reactor and ultrasonic waves were applied as a 0.5sec pulse to prevent mixture heating. A zerotime aliquot (250μl) was removed from the solution prior to reaction launching by simultaneous addition of hydrogen peroxide and application of ultrasonic waves. Hydrogen peroxide was added to obtain a final hydrogen peroxide/heparin (w/w) ratio of 0.15 (3.75 mg/mL).
Ir nge ár hmä dispositivo:
UP50H with sonotrode MS3
Referencia yá 'be̲fi ya nthoni:
Achour, Oussama; Bridiau, Nicolas; Godhbani, Azza; Le Joubioux, Florian; Bordenave Juchereau, Stephanie; Sannier, Fredéric; Piot, Jean-Marie; Fruitier Arnaudin, Ingrid; Maugard, Thierry (2013): Ultrasonic-assisted preparation of a low molecular weight heparin (LMWH) with anticoagulant activity. Carbohydrate Polymers 97; 2013. 684–689.
lúpulo
Nt'ot'e ultrasónica:
Extraction of active compounds / herbal extracts in water and ethanol.
Ir nge ár hmä dispositivo:
UP400S
Extraction of active compounds / herbal extracts in water and ethanol.
Ir nge ár hmä dispositivo:
UP400S
Lactobacillus (lysis/ DNA isolation of various Lactobacillus strains)
Nt'ot'e ultrasónica:
Lactobacillus strains: L. pontis, L. sanfranciscensis, L. farciminis, L. panis, L. oris, L. vaginalis and L. reuteri, L. sp.
Procedure: For the DNA isolation of single colonies, an ultrasonic lysis protocol was developed. One colony (2- to 3-mm diameter) was suspended in 100μl lysis buffer (20 mM EDTA, 10 mM Tris [pH 7.9], 1% Triton X-100, 500 mM guanidine-HCl, 250 mM NaCl). Cells were lysed by 1 min of ultrasonication with an probe-type ultrasonicator UP50H. After the addition of 150μl of cold ethanol (-20°C), the mixture was centrifuged over a spin column of the QIAamp tissue kit and finally eluted with 60μl of buffer (10 mM Tris [pH 7,5]).
To have a tool for a fast and reliable identification of single pure cultures, the PCR assay was combined with a fast DNA isolation procedure. Time-consuming enzymatic lysis procedures and variable susceptibility of bacteria to the lysozyme were overcome by ultrasonic treatment of the cells, with subsequent purification and concentration by binding DNA to a silica matrix. The cell material of a single colony was found to be sufficient for the PCR.
Ir nge ár hmä dispositivo:
UP50H
Referencia yá 'be̲fi ya nthoni:
Müller, M. R. A. (2000): Characterization of the Microbial Ecosystem of Cereal Fermentations using Molecular Biological Methods. Dissertation University of Cologne, 2000.
Lactobacillus strains: L. pontis, L. sanfranciscensis, L. farciminis, L. panis, L. oris, L. vaginalis and L. reuteri, L. sp.
Procedure: For the DNA isolation of single colonies, an ultrasonic lysis protocol was developed. One colony (2- to 3-mm diameter) was suspended in 100μl lysis buffer (20 mM EDTA, 10 mM Tris [pH 7.9], 1% Triton X-100, 500 mM guanidine-HCl, 250 mM NaCl). Cells were lysed by 1 min of ultrasonication with an probe-type ultrasonicator UP50H. After the addition of 150μl of cold ethanol (-20°C), the mixture was centrifuged over a spin column of the QIAamp tissue kit and finally eluted with 60μl of buffer (10 mM Tris [pH 7,5]).
To have a tool for a fast and reliable identification of single pure cultures, the PCR assay was combined with a fast DNA isolation procedure. Time-consuming enzymatic lysis procedures and variable susceptibility of bacteria to the lysozyme were overcome by ultrasonic treatment of the cells, with subsequent purification and concentration by binding DNA to a silica matrix. The cell material of a single colony was found to be sufficient for the PCR.
Ir nge ár hmä dispositivo:
UP50H
Referencia yá 'be̲fi ya nthoni:
Müller, M. R. A. (2000): Characterization of the Microbial Ecosystem of Cereal Fermentations using Molecular Biological Methods. Dissertation University of Cologne, 2000.
Lactobacillus acidophilus Gr+
Nt'ot'e ultrasónica:
Ultrasonic inactivation of Lactobacillus acidophilus Gr+ in milk and juices.
Ir nge ár hmä dispositivo:
UIP500hd
Referencia yá 'be̲fi ya nthoni:
Zenker, M.; Heinz, V.; Knorr, D. (2003): Application of ultrasound assisted thermal processing for preservation and quality retention of liquid foods. J Food Prot 66/2003. pp. 1642–1649.
Ultrasonic inactivation of Lactobacillus acidophilus Gr+ in milk and juices.
Ir nge ár hmä dispositivo:
UIP500hd
Referencia yá 'be̲fi ya nthoni:
Zenker, M.; Heinz, V.; Knorr, D. (2003): Application of ultrasound assisted thermal processing for preservation and quality retention of liquid foods. J Food Prot 66/2003. pp. 1642–1649.
Legionella pneumophila Gr+ in diluted medium
Nt'ot'e ultrasónica:
Ultrasonic inactivation of Legionella pneumophila Gr+ in diluted medium.
Ir nge ár hmä dispositivo:
UIP500hd
Referencia yá 'be̲fi ya nthoni:
Dadjour, M. F.; Ogino, C.; Matsumura, S.; Nakamura, S.; Shimizu N. (2006): Disinfection of Legionella pneumophila by ultrasonic treatment with TiO2. Water Res 40/2006. pp.1137–1142.
Ultrasonic inactivation of Legionella pneumophila Gr+ in diluted medium.
Ir nge ár hmä dispositivo:
UIP500hd
Referencia yá 'be̲fi ya nthoni:
Dadjour, M. F.; Ogino, C.; Matsumura, S.; Nakamura, S.; Shimizu N. (2006): Disinfection of Legionella pneumophila by ultrasonic treatment with TiO2. Water Res 40/2006. pp.1137–1142.
Leuconostoc mesenteroides
Nt'ot'e ultrasónica:
Leukocyte lysozme activity in myelocytic leukemia: the cell suspension was sonicated for 15 min. and samples assayed for lysozyme activity. The lysozyme concentration of the leukocytes ug./10 cells were determined.
Ir nge ár hmä dispositivo:
UP50H
Leukocyte lysozme activity in myelocytic leukemia: the cell suspension was sonicated for 15 min. and samples assayed for lysozyme activity. The lysozyme concentration of the leukocytes ug./10 cells were determined.
Ir nge ár hmä dispositivo:
UP50H
Leukocytic isozym activity in myelocytic leukemia
Nt'ot'e ultrasónica:
Sample preparation: cell suspension was ultrasonically treated and samples assayed for lysozyme activity. The lysozyme concentration of the leukocytes ug/106 was determined.
Ir nge ár hmä dispositivo:
UP200St
Sample preparation: cell suspension was ultrasonically treated and samples assayed for lysozyme activity. The lysozyme concentration of the leukocytes ug/106 was determined.
Ir nge ár hmä dispositivo:
UP200St
Liposomas
Nt'ot'e ultrasónica:
Formation of SUVs
'Yot'e clic nuwa pa da lei mäs dige ar nt'ot'e ultrasónica liposomas.
Ir nge ár hmä dispositivo:
UP50H; 3 cycles of 5 min; in an ice bath.
Formation of SUVs
'Yot'e clic nuwa pa da lei mäs dige ar nt'ot'e ultrasónica liposomas.
Ir nge ár hmä dispositivo:
UP50H; 3 cycles of 5 min; in an ice bath.
Xí malaquita
Nt'ot'e ultrasónica:
Sonophotocatalytic degradation of Malachite Green (a strong bactericide): The photocatalytic degradation alone is considerably faster than sonolytic degradation, efficiency can be improved by coupling the two processes. Malachite green, a strong bactericide, is very ecotoxic to marine bacteria but it is converted to organics that are less or not toxic.
Ir nge ár hmä dispositivo:
UP400S
Sonophotocatalytic degradation of Malachite Green (a strong bactericide): The photocatalytic degradation alone is considerably faster than sonolytic degradation, efficiency can be improved by coupling the two processes. Malachite green, a strong bactericide, is very ecotoxic to marine bacteria but it is converted to organics that are less or not toxic.
Ir nge ár hmä dispositivo:
UP400S
Mangiferin acylation
Nt'ot'e ultrasónica:
Mangiferina (1,3,6,7-tetrahidroxi-2-[3,4,5-trihidroxi-6-(hidroximetil)oxan-2-il]xanteno-9-ona; fórmula: C19H18O11)is a polyphenol of C-glycosylxanthone structure that can be found in many plant species. Mangiferin shows various pharmacological activities. The regioselective acylation of mangiferin can be very efficiently catalyzed by lipase under ultrasonication. Compared with the conventional methods, the ultrasonically-assisted catalysis excels by the advantages of shorter reaction time and higher yields. The optimum conditions for the ultrasonic mangiferin acylation were found as following:
lipasa: PCL, donante acilo: acetato vinilo; disolvente de reacción: DMSO, temperatura de reacción: 45 °C, potencia ultrasónica: 200W; Nthe sustrato: donante acilo yá mangiferina 6 yá 1, carga enzimática: 6 mg yá mL
Rendimiento acilación regioselectiva bí asta ar 84%.
Ir nge ár hmä dispositivo:
UP200St o UP200Ht
Referencia yá 'be̲fi ya nthoni:
cp.: Wang, Z.; Wang, R.; Tian, J.; Zhao, B; Wei, X.F.; Su, Y.L.; Li, C.Y.; Cao, S.G.; Wang, L. (2010): The effect of ultrasound on lipase-catalyzed regioselective acylation of mangiferin in non-aqueous solvents. J. Asian Nat Prod. Res. 12/1, 2010. 56-63.
Mangiferina (1,3,6,7-tetrahidroxi-2-[3,4,5-trihidroxi-6-(hidroximetil)oxan-2-il]xanteno-9-ona; fórmula: C19H18O11)is a polyphenol of C-glycosylxanthone structure that can be found in many plant species. Mangiferin shows various pharmacological activities. The regioselective acylation of mangiferin can be very efficiently catalyzed by lipase under ultrasonication. Compared with the conventional methods, the ultrasonically-assisted catalysis excels by the advantages of shorter reaction time and higher yields. The optimum conditions for the ultrasonic mangiferin acylation were found as following:
lipasa: PCL, donante acilo: acetato vinilo; disolvente de reacción: DMSO, temperatura de reacción: 45 °C, potencia ultrasónica: 200W; Nthe sustrato: donante acilo yá mangiferina 6 yá 1, carga enzimática: 6 mg yá mL
Rendimiento acilación regioselectiva bí asta ar 84%.
Ir nge ár hmä dispositivo:
UP200St o UP200Ht
Referencia yá 'be̲fi ya nthoni:
cp.: Wang, Z.; Wang, R.; Tian, J.; Zhao, B; Wei, X.F.; Su, Y.L.; Li, C.Y.; Cao, S.G.; Wang, L. (2010): The effect of ultrasound on lipase-catalyzed regioselective acylation of mangiferin in non-aqueous solvents. J. Asian Nat Prod. Res. 12/1, 2010. 56-63.
Molecules extraction
Nt'ot'e ultrasónica:
Extraction protocol for extraction from gypsum, rheolite, basalt, edfell ash and obsidian glass:
A 1g sample of regolith or crushed rock is subject to liquid extraction under ultrasonic irradiation to extract and solubilise target molecules. Therefore, 3ml MeOH P80 was added to 1g analogue sample in a glass test tube and sonicated for 20 minutes with an ultrasonic probe-type device UP50H set at 40% amplitude. The mixture was allowed to stand for 10 minutes, and the cloudy supernatant that had formed above the sedimented analogue sample was decanted into 1.5 ml centrifuge tubes. To minimize loss of extracted components by adsorption to surfaces of the hydrophobic polymer centrifuge tubes, the tubes were first blocked with 0.5% (w/v) BSA in 100 mM HEPES pH 7.4. The supernatants were clarified by centrifugation at 17000 G for 10 minutes and stored at 2 – 8°C in glass vials until tested in the immunoassay.
Ir nge ár hmä dispositivo:
UP50H
Referencia yá 'be̲fi ya nthoni:
Rix, C.(2012): Detecting Life on Mars and the Life Marker Chip: Antibody Assays for Detecting Organic Molecules in Liquid Extracts of Martian Samples. Dissertation Cranfield University 2012.
Extraction protocol for extraction from gypsum, rheolite, basalt, edfell ash and obsidian glass:
A 1g sample of regolith or crushed rock is subject to liquid extraction under ultrasonic irradiation to extract and solubilise target molecules. Therefore, 3ml MeOH P80 was added to 1g analogue sample in a glass test tube and sonicated for 20 minutes with an ultrasonic probe-type device UP50H set at 40% amplitude. The mixture was allowed to stand for 10 minutes, and the cloudy supernatant that had formed above the sedimented analogue sample was decanted into 1.5 ml centrifuge tubes. To minimize loss of extracted components by adsorption to surfaces of the hydrophobic polymer centrifuge tubes, the tubes were first blocked with 0.5% (w/v) BSA in 100 mM HEPES pH 7.4. The supernatants were clarified by centrifugation at 17000 G for 10 minutes and stored at 2 – 8°C in glass vials until tested in the immunoassay.
Ir nge ár hmä dispositivo:
UP50H
Referencia yá 'be̲fi ya nthoni:
Rix, C.(2012): Detecting Life on Mars and the Life Marker Chip: Antibody Assays for Detecting Organic Molecules in Liquid Extracts of Martian Samples. Dissertation Cranfield University 2012.
Mouse liver pellets
Nt'ot'e ultrasónica:
The pellets were washed and sonicated for 5 min with a further 0.5 mL of LB2 and centrifuged at 12,000 g for another 20 min, and the resulting two fractions of supernatant were collected. Finally, the pellets were dissolved with 0.5 mL of buffer containing 40 mM tris base, 5 M urea, 2 M thiourea, 4% CHAPS, 100 mM DTT, 0.5% (v/v) biolyte 3-10 (LB3), and were sonicated and centrifuged at 12,000 g for 20 min.
Ir nge ár hmä dispositivo:
UP200S; for 5min.
Referencia yá 'be̲fi ya nthoni:
Gazzana et al. (2009): An update on mouse liver proteome.
The pellets were washed and sonicated for 5 min with a further 0.5 mL of LB2 and centrifuged at 12,000 g for another 20 min, and the resulting two fractions of supernatant were collected. Finally, the pellets were dissolved with 0.5 mL of buffer containing 40 mM tris base, 5 M urea, 2 M thiourea, 4% CHAPS, 100 mM DTT, 0.5% (v/v) biolyte 3-10 (LB3), and were sonicated and centrifuged at 12,000 g for 20 min.
Ir nge ár hmä dispositivo:
UP200S; for 5min.
Referencia yá 'be̲fi ya nthoni:
Gazzana et al. (2009): An update on mouse liver proteome.
Mouse liver suspension
Nt'ot'e ultrasónica:
Sample homogenization to produce cell lysate.
Ir nge ár hmä dispositivo:
UP200S; for 3 x 20 sec.
Referencia yá 'be̲fi ya nthoni:
Gazzana et al. (2009): An update on mouse liver proteome.
Sample homogenization to produce cell lysate.
Ir nge ár hmä dispositivo:
UP200S; for 3 x 20 sec.
Referencia yá 'be̲fi ya nthoni:
Gazzana et al. (2009): An update on mouse liver proteome.
Penicillium digitatum
Nt'ot'e ultrasónica:
Ultrasonic inactivation of Penicillium digitatum (a plant pathogen) in Sabouraud growth medium.
Ir nge ár hmä dispositivo:
UP200St
Referencia yá 'be̲fi ya nthoni:
López-Malo, A.; Palou, E.; Jiménez-Fernández, M.; Alzamora, S. M.; Guerrero, S. (2005): Multifactorial fungal inactivation combining thermosonication and antimicrobials. J. Food Eng. 67/ 2005. pp. 87–93.
Ultrasonic inactivation of Penicillium digitatum (a plant pathogen) in Sabouraud growth medium.
Ir nge ár hmä dispositivo:
UP200St
Referencia yá 'be̲fi ya nthoni:
López-Malo, A.; Palou, E.; Jiménez-Fernández, M.; Alzamora, S. M.; Guerrero, S. (2005): Multifactorial fungal inactivation combining thermosonication and antimicrobials. J. Food Eng. 67/ 2005. pp. 87–93.
Phycocyanin from Spirulina platensis (Arthrospira platensis)
Nt'ot'e ultrasónica:
Extraction of phycocyanin from Spirulina platensis (Arthrospira platensis) cells.
Ir nge ár hmä dispositivo:
UP400S
Extraction of phycocyanin from Spirulina platensis (Arthrospira platensis) cells.
Ir nge ár hmä dispositivo:
UP400S
Plant cells and plant tissue
Nt'ot'e ultrasónica:
30% packed plant cells (W/V) and distilled water are disrupted by sonication for 1-15 min.; plant tissue disintegration: 1 g dried tissue suspended in alcohol is disintegrated during sonication of about 5 min.
Ir nge ár hmä dispositivo:
UP100H
30% packed plant cells (W/V) and distilled water are disrupted by sonication for 1-15 min.; plant tissue disintegration: 1 g dried tissue suspended in alcohol is disintegrated during sonication of about 5 min.
Ir nge ár hmä dispositivo:
UP100H
Plaquetas
Nt'ot'e ultrasónica:
Platelet lysate preparation: Disruption in 1-5 min.
Ir nge ár hmä dispositivo:
UP200Ht
Platelet lysate preparation: Disruption in 1-5 min.
Ir nge ár hmä dispositivo:
UP200Ht
Pleurotus tuberregium
Nt'ot'e ultrasónica:
Extraction of polysaccharides from the edible fungus Pleurotus tuberregium
Ir nge ár hmä dispositivo:
UP400S
Extraction of polysaccharides from the edible fungus Pleurotus tuberregium
Ir nge ár hmä dispositivo:
UP400S
Poly(lactic-co-glycolic acid) (PLGA)
Nt'ot'e ultrasónica:
Preparation of bovine serum albumin (BSA)-loaded poly(lactic-co-glycolic acid) (PLGA)by sonication in 40 sec.
Ir nge ár hmä dispositivo:
Dmini; for 40sec.
Referencia yá 'be̲fi ya nthoni:
Freitas et al. (2005): Flow-through ultrasonic emulsification combined with static micromixing for aseptic production of microspheres by solvent extraction.
Preparation of bovine serum albumin (BSA)-loaded poly(lactic-co-glycolic acid) (PLGA)by sonication in 40 sec.
Ir nge ár hmä dispositivo:
Dmini; for 40sec.
Referencia yá 'be̲fi ya nthoni:
Freitas et al. (2005): Flow-through ultrasonic emulsification combined with static micromixing for aseptic production of microspheres by solvent extraction.
Polyphenols from apple
Nt'ot'e ultrasónica:
Ultrasonic extraction of polyphenols from apple. Solvent: aqueous. Increased yield by 6%; sonication intensity: 20-75Ws/ml; process temp.: heated to 80 degC.
Ir nge ár hmä dispositivo:
UIP2000hd
Referencia yá 'be̲fi ya nthoni:
Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.
Ultrasonic extraction of polyphenols from apple. Solvent: aqueous. Increased yield by 6%; sonication intensity: 20-75Ws/ml; process temp.: heated to 80 degC.
Ir nge ár hmä dispositivo:
UIP2000hd
Referencia yá 'be̲fi ya nthoni:
Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.
Polyphenols from black tea
Nt'ot'e ultrasónica:
Ultrasonic extraction of polyphenols from black tea. Solvent: aqueous. Increased yield by 6-18%; sonication intensity: 8-10Ws/ml; ambient pressure, process temp.: heated to 90 degC.
Ir nge ár hmä dispositivo:
UIP2000hd
Referencia yá 'be̲fi ya nthoni:
Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.
Ultrasonic extraction of polyphenols from black tea. Solvent: aqueous. Increased yield by 6-18%; sonication intensity: 8-10Ws/ml; ambient pressure, process temp.: heated to 90 degC.
Ir nge ár hmä dispositivo:
UIP2000hd
Referencia yá 'be̲fi ya nthoni:
Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.
Polyphenols from red grape marc
Nt'ot'e ultrasónica:
Ultrasonic extraction of polyphenols from red grape marc. Solvent: aqueous. Increased yield by 11-35%; sonication intensity: 20-75Ws/ml; ambient pressure.
Ir nge ár hmä dispositivo:
UIP2000hd
Referencia yá 'be̲fi ya nthoni:
Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.
Ultrasonic extraction of polyphenols from red grape marc. Solvent: aqueous. Increased yield by 11-35%; sonication intensity: 20-75Ws/ml; ambient pressure.
Ir nge ár hmä dispositivo:
UIP2000hd
Referencia yá 'be̲fi ya nthoni:
Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.
Polyphenols, amino acid and caffeine from green tea
Nt'ot'e ultrasónica:
Extraction of active compounds from Green Tea in water
Ir nge ár hmä dispositivo:
UP400S
Extraction of active compounds from Green Tea in water
Ir nge ár hmä dispositivo:
UP400S
Pork: Brining of pork loins
Nt'ot'e ultrasónica:
For ultrasonically assisted brining, pork loins (Longissimus dorsi) were immersed in sodium chloride brine (40 g L−1) and treated at 5°C with low-frequency ultrasound (20 kHz) at low-intensity (2–4 W/cm−2). The effect of the ultrasonic assisted curing on porcine tissue microstructure, protein denaturation, water-binding capacity (WBC), water-holding capacity (WHC), sodium chloride diffusion coefficient (D) and on meat textural profile (TPA) was investigated. Results showed that the ultrasonic treatment caused favourable microstructural changes in meat tissue. Water-holding capacity and textural properties were improved by ultrasonic treatment compared to both tumbled and static brined samples. However, these positive effects were highly dependent on ultrasonic intensity. Higher intensities and/or longer treatment times caused denaturation of proteins. The constant diffusion coefficient model was able to describe precisely the NaCl diffusion kinetics during brining. Ultrasonic treatment significantly enhanced the diffusion of salt compared to samples brined under static conditions and diffusion coefficient exponentially increased with increased ultrasonic intensity.
Ir nge ár hmä dispositivo:
UP200Ht with sonotrode S26d40
Referencia yá 'be̲fi ya nthoni:
Siro, I.; Ven, Cs.; Balla, Cs.; Jonas, G.; Zeke, I.; Friedrich, L. (2009): Application of an ultrasonic assisted curing technique for improving the diffusion of sodium chloride in porcine meat. Journal of Food Engineering 91/2, 2009. 353–362.
For ultrasonically assisted brining, pork loins (Longissimus dorsi) were immersed in sodium chloride brine (40 g L−1) and treated at 5°C with low-frequency ultrasound (20 kHz) at low-intensity (2–4 W/cm−2). The effect of the ultrasonic assisted curing on porcine tissue microstructure, protein denaturation, water-binding capacity (WBC), water-holding capacity (WHC), sodium chloride diffusion coefficient (D) and on meat textural profile (TPA) was investigated. Results showed that the ultrasonic treatment caused favourable microstructural changes in meat tissue. Water-holding capacity and textural properties were improved by ultrasonic treatment compared to both tumbled and static brined samples. However, these positive effects were highly dependent on ultrasonic intensity. Higher intensities and/or longer treatment times caused denaturation of proteins. The constant diffusion coefficient model was able to describe precisely the NaCl diffusion kinetics during brining. Ultrasonic treatment significantly enhanced the diffusion of salt compared to samples brined under static conditions and diffusion coefficient exponentially increased with increased ultrasonic intensity.
Ir nge ár hmä dispositivo:
UP200Ht with sonotrode S26d40
Referencia yá 'be̲fi ya nthoni:
Siro, I.; Ven, Cs.; Balla, Cs.; Jonas, G.; Zeke, I.; Friedrich, L. (2009): Application of an ultrasonic assisted curing technique for improving the diffusion of sodium chloride in porcine meat. Journal of Food Engineering 91/2, 2009. 353–362.
Porphyra yezoensis
Nt'ot'e ultrasónica:
Ultrasonic degradation of polysaccharides from Porphyra yezoensis: 50mL of 1.0 g/100mL porphyra yezoensis polysaccarides (dry weight) solution have been sonicated for 4 hr with an UP400S.
Ir nge ár hmä dispositivo:
UP400S; pulsed ultrasound (cycles: 2 sec on/ 2 sec off) at 20°C.
Ultrasonic degradation of polysaccharides from Porphyra yezoensis: 50mL of 1.0 g/100mL porphyra yezoensis polysaccarides (dry weight) solution have been sonicated for 4 hr with an UP400S.
Ir nge ár hmä dispositivo:
UP400S; pulsed ultrasound (cycles: 2 sec on/ 2 sec off) at 20°C.
polvos
Nt'ot'e ultrasónica:
Grinding, deagglomeration and dispersion to small, relativley uniform particle size.
Ir nge ár hmä dispositivo:
UP200St
Grinding, deagglomeration and dispersion to small, relativley uniform particle size.
Ir nge ár hmä dispositivo:
UP200St
Rat bones
Rat liver
Nt'ot'e ultrasónica:
Disruption and tissue homogenization with an UP400S.
Ir nge ár hmä dispositivo:
UP400S; 3 times for 30 sec.; on ice.
Referencia yá 'be̲fi ya nthoni:
Oh et al. (2003): Acetyl-CoA Carboxylase Gene Is Regulated by Sterol Regulatory Element-binding Protein-1 in Liver. Journal of Biological Chemistry 2003.
Disruption and tissue homogenization with an UP400S.
Ir nge ár hmä dispositivo:
UP400S; 3 times for 30 sec.; on ice.
Referencia yá 'be̲fi ya nthoni:
Oh et al. (2003): Acetyl-CoA Carboxylase Gene Is Regulated by Sterol Regulatory Element-binding Protein-1 in Liver. Journal of Biological Chemistry 2003.
Rat skin
Rawolfia Serpentina
rebaudiósido A
Nt'ot'e ultrasónica:
Samples of 10g dry and ground stevia leaves were extracted in 100mL water under continuous stirring (with a magnetic stirrer). The pH value was controlled with 0.01 M pH 7 sodium phosphate. The sample was put in a 150 mL glass beaker and sonicated with an probe-type ultrasonicator (UIP500hd, 20kHz, 500W). The tip of the sonotrode was immersed about 1.5 cm into the slurry of stevia leaves. The ultrasonic device was set for a power output of 350W. A mild sonication treatment of 350 W for 5-10 min. at a constant process temperature of 30°C gave a rebaudioside A yield of 30-34g per 100g sample. After sonication, the extract solution was centrifuged and filtered off through 0.45 μm microporous membrane; the filtrate was taken for total rebaudioside A content analysis. The extraction yield of total rebaudioside A content was analyzed by HPLC.
Ir nge ar extracción asistida ya ultrasonidos hinda disolventes, bí obtuvo 'nar mar hñets'i rendimiento rebaudiósido A jar comparación ko ya nt'ot'e ar extracción hneise̲, komongu ar extracción ya pa wa ar maceración.
Ir nge ár hmä dispositivo:
UIP500hd
Samples of 10g dry and ground stevia leaves were extracted in 100mL water under continuous stirring (with a magnetic stirrer). The pH value was controlled with 0.01 M pH 7 sodium phosphate. The sample was put in a 150 mL glass beaker and sonicated with an probe-type ultrasonicator (UIP500hd, 20kHz, 500W). The tip of the sonotrode was immersed about 1.5 cm into the slurry of stevia leaves. The ultrasonic device was set for a power output of 350W. A mild sonication treatment of 350 W for 5-10 min. at a constant process temperature of 30°C gave a rebaudioside A yield of 30-34g per 100g sample. After sonication, the extract solution was centrifuged and filtered off through 0.45 μm microporous membrane; the filtrate was taken for total rebaudioside A content analysis. The extraction yield of total rebaudioside A content was analyzed by HPLC.
Ir nge ar extracción asistida ya ultrasonidos hinda disolventes, bí obtuvo 'nar mar hñets'i rendimiento rebaudiósido A jar comparación ko ya nt'ot'e ar extracción hneise̲, komongu ar extracción ya pa wa ar maceración.
Ir nge ár hmä dispositivo:
UIP500hd
Almidón Arros
Nt'ot'e ultrasónica:
Disruption, starch isolation, and break of noncovalent bonds between protein and starch in a rice flour slurry (33%) in 20 – 40 min.
Ir nge ár hmä dispositivo:
UP500hd; 20 – 40 min.
Disruption, starch isolation, and break of noncovalent bonds between protein and starch in a rice flour slurry (33%) in 20 – 40 min.
Ir nge ár hmä dispositivo:
UP500hd; 20 – 40 min.
Rotifers
Nt'ot'e ultrasónica:
Cell disruption of mesozooplankton: by sonication under following conditions of four flow rates (200, 400, 520 and 800lh-1) and four amplitudes (25, 50, 75 and 100%) a killing rate between 58 and 85% has been achieved.
Ir nge ár hmä dispositivo:
UP2000 with flow cell
Referencia yá 'be̲fi ya nthoni:
Viitaslo et al. (2005): Ozone, ultraviolet light, ultrasound and hydrogen peroxide as ballast water treatments – Experiments with mesozooplankton in low-saline brackish water. Journal of Marine Environmental Engineering, 8(1), 35-55.
Cell disruption of mesozooplankton: by sonication under following conditions of four flow rates (200, 400, 520 and 800lh-1) and four amplitudes (25, 50, 75 and 100%) a killing rate between 58 and 85% has been achieved.
Ir nge ár hmä dispositivo:
UP2000 with flow cell
Referencia yá 'be̲fi ya nthoni:
Viitaslo et al. (2005): Ozone, ultraviolet light, ultrasound and hydrogen peroxide as ballast water treatments – Experiments with mesozooplankton in low-saline brackish water. Journal of Marine Environmental Engineering, 8(1), 35-55.
S. fragilis
Saccharomyces cerevisiae
Nt'ot'e ultrasónica:
ruptura
Ir nge ár hmä dispositivo:
UP400S
Referencia yá 'be̲fi ya nthoni:
Guerrero, S,; López-Malo, A.; Alzamora, S. M. (2001): Effect of ultrasound on the survival of Saccharomyces cerevisiae: influence of temperature, pH and amplitude. Innov. Food Sci. Emerg Technol. 2/2001. pp. 31–39.
ruptura
Ir nge ár hmä dispositivo:
UP400S
Referencia yá 'be̲fi ya nthoni:
Guerrero, S,; López-Malo, A.; Alzamora, S. M. (2001): Effect of ultrasound on the survival of Saccharomyces cerevisiae: influence of temperature, pH and amplitude. Innov. Food Sci. Emerg Technol. 2/2001. pp. 31–39.
Saccharomyces cerevisiae
Nt'ot'e ultrasónica:
Ultrasonic inactivation of Saccharomyces cerevisiae in Sabouraud growth medium and in saline.
Ir nge ár hmä dispositivo:
UP400S
Referencia yá 'be̲fi ya nthoni:
Yap, A.; Jiranek, V.; Grbin, P.; Barnes, M.; Bates, D. (2007): Studies on the application of high power ultrasonics for barrel and plank cleaning and disinfection. Austr. NZ Wine Indust. J. 22(3)/ 2007. pp. 96–104.
Ultrasonic inactivation of Saccharomyces cerevisiae in Sabouraud growth medium and in saline.
Ir nge ár hmä dispositivo:
UP400S
Referencia yá 'be̲fi ya nthoni:
Yap, A.; Jiranek, V.; Grbin, P.; Barnes, M.; Bates, D. (2007): Studies on the application of high power ultrasonics for barrel and plank cleaning and disinfection. Austr. NZ Wine Indust. J. 22(3)/ 2007. pp. 96–104.
Saffron, crocus sativus
Nt'ot'e ultrasónica:
Extraction of active compounds (flavors and color agents).
Click here to read more about the extraction from saffron!
Ir nge ár hmä dispositivo:
UP50H
Referencia yá 'be̲fi ya nthoni:
Kadkhodaee et al. (2007): Ultrasonic Extraction of Active Compounds from Saffron. Acta Hortic. 739, 2007. 417-425.
Extraction of active compounds (flavors and color agents).
Click here to read more about the extraction from saffron!
Ir nge ár hmä dispositivo:
UP50H
Referencia yá 'be̲fi ya nthoni:
Kadkhodaee et al. (2007): Ultrasonic Extraction of Active Compounds from Saffron. Acta Hortic. 739, 2007. 417-425.
Salmonella Senftenberg 775W Gr-
Nt'ot'e ultrasónica:
Ultrasonic inactivation of Salmonella Senftenberg 775W Gr- in McIlvaine citrate-phosphate buffer or nutrient broth.
Ir nge ár hmä dispositivo:
UP100H
Referencia yá 'be̲fi ya nthoni:
Álvarez, I.; Manas, P.; Virto, R.; Condón, S. (2006): Inactivation of Salmonella Senftenberg 775W by ultrasonic waves under pressure at different water activities. Int. J. Food Microbiol. 108/2006. pp. 218–225.
Ultrasonic inactivation of Salmonella Senftenberg 775W Gr- in McIlvaine citrate-phosphate buffer or nutrient broth.
Ir nge ár hmä dispositivo:
UP100H
Referencia yá 'be̲fi ya nthoni:
Álvarez, I.; Manas, P.; Virto, R.; Condón, S. (2006): Inactivation of Salmonella Senftenberg 775W by ultrasonic waves under pressure at different water activities. Int. J. Food Microbiol. 108/2006. pp. 218–225.
Salvia miltiorrhiza bunge
Nt'ot'e ultrasónica:
Extraction of biological active compounds: sodium Danshensu and four tanshinones (dihydrotanshione I, tanshinone I, cryptotanshinone and tanshinone IIA).
Ir nge ár hmä dispositivo:
UP100H
Extraction of biological active compounds: sodium Danshensu and four tanshinones (dihydrotanshione I, tanshinone I, cryptotanshinone and tanshinone IIA).
Ir nge ár hmä dispositivo:
UP100H
Salvia Officinalis
Nt'ot'e ultrasónica:
Extraction of active compounds from Salvia Officinalis (sage) in less than 2hr.
Ir nge ár hmä dispositivo:
UP50H
Extraction of active compounds from Salvia Officinalis (sage) in less than 2hr.
Ir nge ár hmä dispositivo:
UP50H
suero
Sheep cystic livers, lungs and positive blood (Echinococcus granulosus antigens)
Nt'ot'e ultrasónica:
Sheep cystic livers, lungs and positive blood (Echinococcus granulosus antigens in lambs): The sample was then sonicated for 2 × 15 sec on ice until no intact protoscolices were visible.
Ir nge ár hmä dispositivo:
UP200S; 2 x 15 sec.
Referencia yá 'be̲fi ya nthoni:
Tabar et al. (2009): Antibody response against hydatid fluid, protoscolex and whole body of Echinococcus granulosus antigens in lambs. Journal of Veterinary Research, Volume 10, Issue 3; 2009. 283-288.
Sheep cystic livers, lungs and positive blood (Echinococcus granulosus antigens in lambs): The sample was then sonicated for 2 × 15 sec on ice until no intact protoscolices were visible.
Ir nge ár hmä dispositivo:
UP200S; 2 x 15 sec.
Referencia yá 'be̲fi ya nthoni:
Tabar et al. (2009): Antibody response against hydatid fluid, protoscolex and whole body of Echinococcus granulosus antigens in lambs. Journal of Veterinary Research, Volume 10, Issue 3; 2009. 283-288.
Sorbitant Trioleate, ethanol (as solvent) and Bi-2212 powder
Nt'ot'e ultrasónica:
Deagglomerate a slurry containing the dispersant Sorbitant Trioleate, ethanol (as solvent) and Bi-2212 powder.
Ir nge ár hmä dispositivo:
UP200S; for 3 min.
Referencia yá 'be̲fi ya nthoni:
Mora et al. (2009): Fabrication of Superconducting Coatings on Structural Ceramic Tiles.
Deagglomerate a slurry containing the dispersant Sorbitant Trioleate, ethanol (as solvent) and Bi-2212 powder.
Ir nge ár hmä dispositivo:
UP200S; for 3 min.
Referencia yá 'be̲fi ya nthoni:
Mora et al. (2009): Fabrication of Superconducting Coatings on Structural Ceramic Tiles.
Soy isoflavones
Nt'ot'e ultrasónica:
ultrasonic extraction of soy isoflavones in water and solvent. Increased yield of up to 15% in extraction efficiency.
Ir nge ár hmä dispositivo:
UP200St
Referencia yá 'be̲fi ya nthoni:
Rostagno et al. (2003), referenced by Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.
ultrasonic extraction of soy isoflavones in water and solvent. Increased yield of up to 15% in extraction efficiency.
Ir nge ár hmä dispositivo:
UP200St
Referencia yá 'be̲fi ya nthoni:
Rostagno et al. (2003), referenced by Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.
Proteína soja
Nt'ot'e ultrasónica:
Ultrasonic extraction of soy protein in water and alkali (sodium hydroxide). Increased yield by 53%. Inline sonication was found more efficient as batch extraction (inline sonication gave a 23% higher yield than batch sonication).
Ir nge ár hmä dispositivo:
UIP1000hd for batch/ beaker and with flow cell reactor for inline sonication.
Referencia yá 'be̲fi ya nthoni:
Moulton and Wang (1982), referenced by Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.
Ultrasonic extraction of soy protein in water and alkali (sodium hydroxide). Increased yield by 53%. Inline sonication was found more efficient as batch extraction (inline sonication gave a 23% higher yield than batch sonication).
Ir nge ár hmä dispositivo:
UIP1000hd for batch/ beaker and with flow cell reactor for inline sonication.
Referencia yá 'be̲fi ya nthoni:
Moulton and Wang (1982), referenced by Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.
Sperm heads (human)
Sperm tails (human)
Stevia rebaudiana Bert.
Nt'ot'e ultrasónica:
Ultrasonic extraction of stevioside glycoside from samples of 10 g of dry leaves from Stevia rebaudiana with four particles sizes (0.315 mm, 2 mm, 6.3 mm and crushed dry leaves) were mixed with different solvents: distilled water and water/ethanol mixtures (55% and 70%) with different sample weight to solvent volume ratios: 1/10, 1/8, 1/5 (w/v) then exposed to ultrasound at room temperature. Sonication time: < 5 min. Ir nge ár hmä dispositivo:
UP200St
Ultrasonic extraction of stevioside glycoside from samples of 10 g of dry leaves from Stevia rebaudiana with four particles sizes (0.315 mm, 2 mm, 6.3 mm and crushed dry leaves) were mixed with different solvents: distilled water and water/ethanol mixtures (55% and 70%) with different sample weight to solvent volume ratios: 1/10, 1/8, 1/5 (w/v) then exposed to ultrasound at room temperature. Sonication time: < 5 min. Ir nge ár hmä dispositivo:
UP200St
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Nuna tutorial gi mä Temu̲ ar klase ar sonicador ge ar mäs xi hño pa yá tareas ya nt'ot'e muestras, komongu ar lisis, ar disrupción celular, aislamiento proteínas, ar fragmentación ya ADN ne ya ARN jar laboratorios, análisis ne nthoni. Elija ar klase ar sonicador ideal pa ár nt'ot'e, volumen muestra, ya 'bede ya muestra ne ya rendimiento. Hielscher Ultrasonics pe̲ts'i ar homogeneizador ultrasónico ideal pa gí.
Tema tingigi mbo jar sonicador perfecto pa ar disrupción ar celular ne ar extracción proteínas jar ciencia ne análisis
High Performance Ultrasonicators for Any Size
Hielscher Ultrasonics cell disruptors and tissue homogenizers are available at any size for any volume. Whether you have to sonicate small sample sizes, mass samples such 96-well plates, mid-size volumes or truckloads per hour, our portfolio offer the ideal ultrasonicator for your application. Compact, hand-held ultrasonic homogenizers are optimal for lab and research, whilst our industrial series covers everything between 0,5kW to 16kW per ultrasonic processor. With the capability of being installed as clusters, with Hielscher ultrasonicators you can process virtually any volume. The robustness of Hielscher’s ultrasonic equipment allows for 24/7 operation at heavy duty and in demanding environments.
The sophisticated and smart software allows for highest process control and operator’s convenience. All sonication data are automatically recorded on a built-in SD-card. Other smart features include pre-setting and saving of sonication parameters, LAN connection, and browser remote control.
The table below gives you an indication of the approximate processing capacity of our lab-size sonicators for tissue homogenization and other sample preparation tasks:
| Dispositivos recomendados | Volumen lote | Gasto |
|---|---|---|
| UIP400MTP Sonicador ar placas 96 pocillos | Placas multipocillo yá ar microtitulación | n.d. |
| cupHorn ultrasónico | CupHorn pa viales wa ya Baso ar precipitados | n.d. |
| GDmini2 | reactor ultrasónico microflujo | n.d. |
| VialTweeter | 0.5 1.5mL | n.d. |
| UP100H | Ar 1 jar 500 ml | Ar 10 200 ml yá min |
| UP200Ht, UP200St | Ar 10 da 1000 ml | Ar 20 200 ml yá min |
| UP400St | Ar 10 da 2000 ml | Ar 20 400 ml yá min |
| Tamizadora ultrasónica | n.d. | n.d. |
ultrasonicador UP200Ht ko micropunta S26d2 ar 2 mm pa ar sonicación muestras t'olo