Search results for: stem cell
Adipose-derived stem cells (ASCs) are mesenchymal stem cells (MSCs) that are obtained from human adipose tissue. The stem cells in the eviscerated adipose tissue (the so-called stromal vascular fraction (SVF)) must be isolated to use them subsequently for medical or cosmetic treatments. Ultrasonication is a highly effective and reliable treatment to isolate stem cells from adipose tissue. The advantage of ultrasonic stem cell isolation lies in the fact that the ultrasonic treatment is based on the working principle of ultrasonic-derived cavitation, which is mechanical shear. Not chemical or enzymatic additives are required. The ultrasonic stem cell isolation allows for a repeatable protocol, high stem cell yield as well as superior stem cell quality. Hielscher Ultrasonics offers various ultrasonic systems for the direct and indirect sonication of SVF and the release of highly functional stem cells.
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Ultrasonic Stem Cell Isolation
Ultrasonic cavitation is a highly effective mechanical method to isolate stem cells from human adipose tissue. Ultrasonically isolated stromal vascular fraction (SVF) show a high regenerative potential for medical applications. Hielscher Ultrasonics offers various options of direct and indirect sonication…
https://www.hielscher.com/ultrasonic-stem-cell-isolation.htm
Ultrasonic Lysis of Bioengineered Cells in Industrial Production
Bioengineered bacteria species such E. coli as well as genetically modified mammalian and plant cell types are used widely in biotech to express molecules. In order to release these synthesized bio-molecules, a reliable cell disruption technique is required. High-performance ultrasonication…
https://www.hielscher.com/ultrasonic-lysis-of-bioengineered-cells-in-industrial-production.htm
Sonication for Lysis: Cell Disruption and Extraction
Cell disintegration or lysis is a common part of daily sample preparation in biotech laboratories. The goal of lysis is to disrupt parts of the cell wall or the complete cell to release biological molecules. Ultrasonic homogenizers are widely used…
https://www.hielscher.com/ultrasonic-lysis-cell-disruption-extraction.htm
Ultrasonication to Improve Algae Cell Disruption and Extraction
Algae, macro- and microalgae, contain many valuable compounds, which are used as nutritional foods, food additives or as fuel or fuel feedstock. In order to release the target substances from the algae cell a potent and efficient cell disruption technique…
https://www.hielscher.com/ultrasonication-to-improve-algae-cell-disruption-and-extraction.htm
Ultrasonic Protein Extraction from Tissue and Cell Cultures
Protein extraction is an essential sample preparation step in proteomics. Proteins can be extracted from plant and animal tissue, yeasts and microorganisms. Sonication is a reliable, efficient protein extraction method giving high protein yields within a short extraction time. Protein…
https://www.hielscher.com/ultrasonic-protein-extraction-from-tissue-and-cell-cultures.htm
Ultrasonic Mini Flow Cell
Usually intensive ultrasound is transmitted into small volumes via a titanium sonotrode immersed into the liquid. This method is very efficient but has the side effect that there is also cavitation at the sonotrode, which results in a small contamination…
https://www.hielscher.com/dmini_p.htm
Ultrasonic Disintegrators
Cell disintegration is a common task in biotechnology and in many applications that involve processing cells and intracellular material. Ultrasonic cell disintegrators are efficient and reliable, when it comes to breaking cell wall or membranes of plant cells, mammalian tissue,…
https://www.hielscher.com/ultrasonic-disintegrators.htm
How To Use Hielscher’s Ultrasonic Tissue Homogenizers
Before purification or characterization of intracellular macromolecules such as proteins, organelles, enzymes or active compounds, an efficient method for tissue lysis and cell disintegration is required. Ultrasonication is a effective method for cell preparation that releases the intracellular material from…
https://www.hielscher.com/how-to-use-hielschers-ultrasonic-tissue-homogenizers.htm
Cell Lysis of BL21 Cells by Ultrasonication
BL21 cells are a strain of E. coli that is widely used in research laboratories, biotechnology and industrial production due to their capability to express proteins highly efficient. Ultrasonic cell disruption, lysis and protein extraction is the common method to…
https://www.hielscher.com/cell-lysis-of-bl21-cells-by-ultrasonication.htm
MultiPhaseCavitator-Insert for Ultrasonic Flow Cell
The MultiPhaseCavitator Insert (InsertMPC48) is designed to improve the ultrasonic processing of liquid/liquid or liquid/gas mixes. 48 very fine cannulas inject liquid or gas into a liquid phase right in the cavitation zone. This creates very small suspended droplets or…
https://www.hielscher.com/multiphasecavitator-insert-for-ultrasonic-flow-cell-reactor.htm
Ultrasonic Lysis of E. Coli
E. coli bacteria are the most commonly used bacteria in microbiology and biotechnology. Ultrasonic cell disruptors deliver reliable and reproducible results for the lysis of E. coli. Intense yet precisely controllable cavitation and shear forces result in complete disruption and…
https://www.hielscher.com/ultrasonic-lysis-of-e-coli.htm
Sample Preparation with the Ultrasonic VialTweeter
Sample preparation before analysis can require various pre-analytical processes such as tissue homogenization, lysis, extraction of proteins, DNA, RNA, organelles and other intracellular substances, dissolving and degassing. The VialTweeter is a unique ultrasonic device that prepares multiple sample tubes simultaneously…
https://www.hielscher.com/sample-preparation-with-the-ultrasonic-vialtweeter.htm